2y32

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[[Image:2y32.png|left|200px]]
 
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==Crystal structure of bradavidin==
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The line below this paragraph, containing "STRUCTURE_2y32", creates the "Structure Box" on the page.
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<StructureSection load='2y32' size='340' side='right'caption='[[2y32]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2y32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y32 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y32 OCA], [https://pdbe.org/2y32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y32 RCSB], [https://www.ebi.ac.uk/pdbsum/2y32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y32 ProSAT]</span></td></tr>
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{{STRUCTURE_2y32| PDB=2y32 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q89IH6_BRADU Q89IH6_BRADU]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bradavidin is a homotetrameric biotin-binding protein from Bradyrhizobium japonicum, a nitrogen fixing and root nodule-forming symbiotic bacterium of the soybean. Wild-type (wt) bradavidin has 138 amino acid residues, whereas the C-terminally truncated core-bradavidin has only 118 residues. We have solved the X-ray structure of wt bradavidin and found that the C-terminal amino acids of each subunit were uniquely bound to the biotin-binding pocket of an adjacent subunit. The biotin-binding pocket occupying peptide (SEKLSNTK) was named "Brad-tag" and it serves as an intrinsic stabilizing ligand in wt bradavidin. The binding of Brad-tag to core-bradavidin was analysed by isothermal titration calorimetry and a binding affinity of approximately 25 microM was measured. In order to study the potential of Brad-tag, a green fluorescent protein tagged with Brad-tag was prepared and successfully concentrated from a bacterial cell lysate using core-bradavidin-functionalized Sepharose resin.
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===Crystal structure of bradavidin===
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Structure of bradavidin - C-terminal residues act as intrinsic ligands.,Leppiniemi J, Gronroos T, Maatta JA, Johnson MS, Kulomaa MS, Hytonen VP, Airenne TT PLoS One. 2012;7(5):e35962. Epub 2012 May 4. PMID:22574129<ref>PMID:22574129</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2y32" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22574129 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22574129}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2y32]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y32 OCA].
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==Reference==
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<ref group="xtra">PMID:022574129</ref><references group="xtra"/>
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[[Category: Bradyrhizobium japonicum]]
[[Category: Bradyrhizobium japonicum]]
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[[Category: Airenne, T T.]]
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[[Category: Large Structures]]
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[[Category: Gronroos, T.]]
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[[Category: Airenne TT]]
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[[Category: Hytonen, V P.]]
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[[Category: Gronroos T]]
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[[Category: Johnson, M S.]]
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[[Category: Hytonen VP]]
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[[Category: Kulomaa, M S.]]
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[[Category: Johnson MS]]
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[[Category: Leppiniemi, J.]]
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[[Category: Kulomaa MS]]
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[[Category: Biotin-binding protein]]
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[[Category: Leppiniemi J]]

Current revision

Crystal structure of bradavidin

PDB ID 2y32

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