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3vm5

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[[Image:3vm5.jpg|left|200px]]
 
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==Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris==
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The line below this paragraph, containing "STRUCTURE_3vm5", creates the "Structure Box" on the page.
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<StructureSection load='3vm5' size='340' side='right'caption='[[3vm5]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3vm5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VM5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_3vm5| PDB=3vm5 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vm5 OCA], [https://pdbe.org/3vm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vm5 RCSB], [https://www.ebi.ac.uk/pdbsum/3vm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vm5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMY_ORYLA AMY_ORYLA] Catalyzes the hydrolysis of alpha-1,4 glycosidic linkages in starch, glycogen and similar oligosaccharides.<ref>PMID:22613096</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The medaka fish alpha-amylase was expressed and purified. The expression systems were constructed using methylotrophic yeast Pichia pastoris, and the recombinant proteins were secreted into the culture medium. Purified recombinant alpha-amylase exhibited starch hydrolysis activity. The optimal pH, denaturation temperature, and K(M) and V(max) values were determined; chloride ions were essential for enzyme activity. The purified protein was also crystallized and examined by X-ray crystallography. The structure has the (alpha/beta)(8) barrel fold, as do other known alpha-amylases, and the overall structure is very similar to the structure of vertebrate (human and pig) alpha-amylases. A novel expression plasmid was developed. Using this plasmid, high-throughput construction of an expression system by homologous recombination in P. pastoris cells, previously reported for membrane proteins, was successfully applied to the secretory protein.
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===Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris===
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Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris.,Mizutani K, Toyoda M, Otake Y, Yoshioka S, Takahashi N, Mikami B Biochim Biophys Acta. 2012 May 18. PMID:22613096<ref>PMID:22613096</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vm5" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22613096}}, adds the Publication Abstract to the page
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*[[Amylase 3D structures|Amylase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22613096 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22613096}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3vm5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VM5 OCA].
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==Reference==
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<ref group="xtra">PMID:022613096</ref><references group="xtra"/>
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[[Category: Oryzias latipes]]
[[Category: Oryzias latipes]]
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[[Category: Mikami, B.]]
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[[Category: Mikami B]]
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[[Category: Mizutani, K.]]
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[[Category: Mizutani K]]
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[[Category: Toyoda, M.]]
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[[Category: Toyoda M]]
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[[Category: Hydrolase]]
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[[Category: Starch hydrolysis]]
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Current revision

Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris

PDB ID 3vm5

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