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4dg7

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[[Image:4dg7.jpg|left|200px]]
 
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==Low resolution structure of Drosophila Translin==
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The line below this paragraph, containing "STRUCTURE_4dg7", creates the "Structure Box" on the page.
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<StructureSection load='4dg7' size='340' side='right'caption='[[4dg7]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4dg7]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DG7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.195&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dg7 OCA], [https://pdbe.org/4dg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dg7 RCSB], [https://www.ebi.ac.uk/pdbsum/4dg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dg7 ProSAT]</span></td></tr>
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{{STRUCTURE_4dg7| PDB=4dg7 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7JVK6_DROME Q7JVK6_DROME]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystals of native Drosophila melanogaster translin diffracted to 7 A resolution. Reductive methylation of the protein improved crystal quality. The native and methylated proteins showed similar profiles in size-exclusion chromatography analyses but the methylated protein displayed reduced DNA-binding activity. Crystals of the methylated protein diffracted to 4.2 A resolution at BM14 of the ESRF synchrotron. Crystals with 49% solvent content belonged to monoclinic space group P21 with eight protomers in the asymmetric unit. Only 2% of low-resolution structures with similar low percentage solvent content were found in the PDB. The crystal structure, solved by molecular replacement method, refined to R work (R free) of 0.24 (0.29) with excellent stereochemistry. The crystal structure clearly shows that drosophila protein exists as an octamer, and not as a decamer as expected from gel-filtration elution profiles. The similar octameric quaternary fold in translin orthologs and in translin-TRAX complexes suggests an up-down dimer as the basic structural subunit of translin-like proteins. The drosophila oligomer displays asymmetric assembly and increased radius of gyration that accounts for the observed differences between the elution profiles of human and drosophila proteins on gel-filtration columns. This study demonstrates clearly that low-resolution X-ray structure can be useful in understanding complex biological oligomers.
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===Low resolution structure of Drosophila Translin===
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Low-resolution structure of Drosophila translin.,Kumar V, Gupta GD FEBS Open Bio. 2012 Mar 15;2:37-46. doi: 10.1016/j.fob.2012.03.001. Print 2012. PMID:23650579<ref>PMID:23650579</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18647346}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 4dg7" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18647346 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18647346}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[4dg7]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DG7 OCA].
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==Reference==
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<ref group="xtra">PMID:018647346</ref><references group="xtra"/>
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Gupta, G D.]]
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[[Category: Large Structures]]
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[[Category: Kumar, V.]]
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[[Category: Gupta GD]]
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[[Category: Dimethylamino-borane]]
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[[Category: Kumar V]]
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[[Category: Dna binding protein]]
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[[Category: Formaldehyde]]
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[[Category: Reductive methylation]]
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[[Category: Ssdna/rna binding]]
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[[Category: Translin-like fold]]
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Current revision

Low resolution structure of Drosophila Translin

PDB ID 4dg7

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