2aff

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2aff" [edit=sysop:move=sysop])
Current revision (07:48, 30 October 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2aff.png|left|200px]]
 
-
<!--
+
==The solution structure of the Ki67FHA/hNIFK(226-269)3P complex==
-
The line below this paragraph, containing "STRUCTURE_2aff", creates the "Structure Box" on the page.
+
<StructureSection load='2aff' size='340' side='right'caption='[[2aff]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2aff]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AFF FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 100 models</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
-
{{STRUCTURE_2aff| PDB=2aff | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aff OCA], [https://pdbe.org/2aff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aff RCSB], [https://www.ebi.ac.uk/pdbsum/2aff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aff ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KI67_HUMAN KI67_HUMAN] Thought to be required for maintaining cell proliferation.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2aff_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aff ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The forkhead-associated (FHA) domain of human Ki67 interacts with the human nucleolar protein hNIFK, recognizing a 44-residue fragment, hNIFK226-269, phosphorylated at Thr234. Here we show that high-affinity binding requires sequential phosphorylation by two kinases, CDK1 and GSK3, yielding pThr238, pThr234 and pSer230. We have determined the solution structure of Ki67FHA in complex with the triply phosphorylated peptide hNIFK226-269(3P), revealing not only local recognition of pThr234 but also the extension of the beta-sheet of the FHA domain by the addition of a beta-strand of hNIFK. The structure of an FHA domain in complex with a biologically relevant binding partner provides insights into ligand specificity and potentially links the cancer marker protein Ki67 to a signaling pathway associated with cell fate specification.
-
===The solution structure of the Ki67FHA/hNIFK(226-269)3P complex===
+
Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67.,Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD Nat Struct Mol Biol. 2005 Nov;12(11):987-93. PMID:16244663<ref>PMID:16244663</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16244663}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2aff" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16244663 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16244663}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
[[2aff]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFF OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:016244663</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Byeon, I J.L.]]
+
[[Category: Large Structures]]
-
[[Category: Gronenborn, A M.]]
+
[[Category: Byeon I-JL]]
-
[[Category: Li, H.]]
+
[[Category: Gronenborn AM]]
-
[[Category: Song, H.]]
+
[[Category: Li H]]
-
[[Category: Tsai, M D.]]
+
[[Category: Song H]]
-
[[Category: Cell cycle]]
+
[[Category: Tsai MD]]
-
[[Category: Fha]]
+
-
[[Category: Ki67]]
+
-
[[Category: Nifk]]
+
-
[[Category: Nmr]]
+
-
[[Category: Phosphoprotein]]
+

Current revision

The solution structure of the Ki67FHA/hNIFK(226-269)3P complex

PDB ID 2aff

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2aff"
Personal tools