3cb4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3cb4" [edit=sysop:move=sysop])
Current revision (08:52, 30 October 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3cb4.png|left|200px]]
 
-
<!--
+
==The Crystal Structure of LepA==
-
The line below this paragraph, containing "STRUCTURE_3cb4", creates the "Structure Box" on the page.
+
<StructureSection load='3cb4' size='340' side='right'caption='[[3cb4]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3cb4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CB4 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cb4 OCA], [https://pdbe.org/3cb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cb4 RCSB], [https://www.ebi.ac.uk/pdbsum/3cb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cb4 ProSAT]</span></td></tr>
-
{{STRUCTURE_3cb4| PDB=3cb4 | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/LEPA_ECOLI LEPA_ECOLI] Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.<ref>PMID:17110332</ref> <ref>PMID:20045415</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/3cb4_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cb4 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
LepA is a highly conserved elongation factor that promotes the back translocation of tRNAs on the ribosome during the elongation cycle. We have determined the crystal structure of LepA from Escherichia coli at 2.8-A resolution. The high degree of sequence identity between LepA and EF-G is reflected in the structural similarity between the individual homologous domains of LepA and EF-G. However, the orientation of domains III and V in LepA differs from their orientations in EF-G. LepA also contains a C-terminal domain (CTD) not found in EF-G that has a previously unobserved protein fold. The high structural similarity between LepA and EF-G enabled us to derive a homology model for LepA bound to the ribosome using a 7.3-A cryo-EM structure of a complex between EF-G and the 70S ribosome. In this model, the very electrostatically positive CTD of LepA is placed in the direct vicinity of the A site of the large ribosomal subunit, suggesting a possible interaction between the CTD and the back translocated tRNA or 23S rRNA.
-
===The Crystal Structure of LepA===
+
The structure of LepA, the ribosomal back translocase.,Evans RN, Blaha G, Bailey S, Steitz TA Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4673-8. Epub 2008 Mar 24. PMID:18362332<ref>PMID:18362332</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_18362332}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3cb4" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 18362332 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_18362332}}
+
-
 
+
-
==About this Structure==
+
-
[[3cb4]] is a 6 chain structure of [[GTP-binding protein]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CB4 OCA].
+
==See Also==
==See Also==
-
*[[GTP-binding protein|GTP-binding protein]]
+
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
-
 
+
*[[Escherichia coli LepA%2C the ribosomal back translocase|Escherichia coli LepA%2C the ribosomal back translocase]]
-
==Reference==
+
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
-
<ref group="xtra">PMID:018362332</ref><references group="xtra"/>
+
== References ==
-
[[Category: Escherichia coli]]
+
<references/>
-
[[Category: Bailey, S.]]
+
__TOC__
-
[[Category: Blaha, G.]]
+
</StructureSection>
-
[[Category: Evans, R N.]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Steitz, T A.]]
+
[[Category: Large Structures]]
-
[[Category: Gtp-binding]]
+
[[Category: Bailey S]]
-
[[Category: Gtpase]]
+
[[Category: Blaha G]]
-
[[Category: Membrane]]
+
[[Category: Evans RN]]
-
[[Category: Nucleotide-binding]]
+
[[Category: Steitz TA]]
-
[[Category: Ob-fold]]
+
-
[[Category: Translation]]
+

Current revision

The Crystal Structure of LepA

PDB ID 3cb4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cb4"
Personal tools