4d8e

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[[Image:4d8e.png|left|200px]]
 
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==High resolution structures of monomeric S. pyogenes SpeB reveals role of glycine-rich active site loop==
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The line below this paragraph, containing "STRUCTURE_4d8e", creates the "Structure Box" on the page.
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<StructureSection load='4d8e' size='340' side='right'caption='[[4d8e]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4d8e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_ATCC_10782 Streptococcus pyogenes ATCC 10782]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D8E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.497&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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{{STRUCTURE_4d8e| PDB=4d8e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d8e OCA], [https://pdbe.org/4d8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d8e RCSB], [https://www.ebi.ac.uk/pdbsum/4d8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d8e ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cysteine protease SpeB is secreted from Streptococcus pyogenes and has been studied as a potential virulence factor since its identification almost 70 years ago. Here, we report the crystal structures of apo mature SpeB to 1.06 Angstrom resolution as well as complexes with the general cysteine protease inhibitor E64 and a novel substrate mimetic peptide inhibitor. These structures uncover conformational changes associated with maturation of SpeB from the inactive zymogen to its active form and identify the residues required for substrate binding. With the use of a newly developed fluorogenic tri-peptide substrate to measure SpeB activity, we determined IC50 values for E64 and our new peptide inhibitor and the effects of mutations within the C-terminal, active-site loop. The structures and mutational analysis suggest conformational movements of the glycine-rich C-terminal loop are important for the recognition and recruitment of biological substrates and release of hydrolyzed products.
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===High resolution structures of monomeric S. pyogenes SpeB reveals role of glycine-rich active site loop===
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Ultra-high and high resolution structures and mutational analysis of monomeric Streptococcus pyogenes SpeB reveal a functional role for the glycine-rich C-terminal loop.,Gonzalez-Paez GE, Wolan DW J Biol Chem. 2012 May 29. PMID:22645124<ref>PMID:22645124</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4d8e" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22645124 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22645124}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[4d8e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D8E OCA].
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[[Category: Streptococcus pyogenes ATCC 10782]]
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[[Category: Gonzalez GE]]
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==Reference==
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[[Category: Wolan DW]]
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<ref group="xtra">PMID:022645124</ref><references group="xtra"/>
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[[Category: Streptococcus pyogenes]]
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[[Category: Streptopain]]
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[[Category: Gonzalez, G E.]]
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[[Category: Wolan, D W.]]
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[[Category: Cysteine protease]]
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[[Category: E64 complex]]
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[[Category: Hydrolase]]
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[[Category: Papain fold]]
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[[Category: Secreted]]
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Current revision

High resolution structures of monomeric S. pyogenes SpeB reveals role of glycine-rich active site loop

PDB ID 4d8e

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