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3t2x

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[[Image:3t2x.png|left|200px]]
 
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==Structure of shwanavidin low affinity mutant (F43A)==
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The line below this paragraph, containing "STRUCTURE_3t2x", creates the "Structure Box" on the page.
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<StructureSection load='3t2x' size='340' side='right'caption='[[3t2x]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3t2x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shedo Shedo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T2X FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3szh|3szh]], [[3szi|3szi]], [[3szj|3szj]], [[3t2w|3t2w]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sden_0912 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=318161 SHEDO])</td></tr>
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{{STRUCTURE_3t2x| PDB=3t2x | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2x OCA], [https://pdbe.org/3t2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t2x RCSB], [https://www.ebi.ac.uk/pdbsum/3t2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t2x ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Shwanavidin is an avidin-like protein from the marine proteobactrium Shewanella denitrificans, which exhibits an innate dimeric structure while maintaining high affinity towards biotin. A unique residue (Phe-43) from the L3,4 loop and a distinctive disulfide bridge were shown to account for the high affinity towards biotin. Phe-43 emulates the function and position of the critical intermonomeric Trp that characterizes the tetrameric avidins but is lacking in shwanavidin. The 18 copies of the apo monomer revealed distinctive snapshots of L3,4 and Phe-43, providing rare insight into loop flexibility, binding-site accessibility and psychrophilic adaptation. Nevertheless, as in all avidins, shwanavidin also displays high thermostability properties. The unique features of shwanavidin may provide a platform for the design of a long-sought-after monovalent form of avidin, which would be ideal for novel types of biotechnological application.
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===Structure of shwanavidin low affinity mutant (F43A)===
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Structural Adaptation of a Thermostable Biotin-Binding Protein in a Psychrophilic Environment.,Meir A, Bayer EA, Livnah O J Biol Chem. 2012 Apr 5. PMID:22493427<ref>PMID:22493427</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3t2x" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22493427 is the PubMed ID number.
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{{ABSTRACT_PUBMED_22493427}}
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==About this Structure==
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[[3t2x]] is a 4 chain structure of [[Avidin]] with sequence from [http://en.wikipedia.org/wiki/Shewanella_denitrificans Shewanella denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2X OCA].
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==See Also==
==See Also==
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*[[Avidin|Avidin]]
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*[[Avidin 3D structures|Avidin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022493427</ref><references group="xtra"/>
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__TOC__
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[[Category: Shewanella denitrificans]]
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</StructureSection>
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[[Category: Livnah, O.]]
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[[Category: Large Structures]]
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[[Category: Meir, A.]]
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[[Category: Shedo]]
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[[Category: Livnah, O]]
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[[Category: Meir, A]]
[[Category: Avidin]]
[[Category: Avidin]]
[[Category: Biotin]]
[[Category: Biotin]]

Current revision

Structure of shwanavidin low affinity mutant (F43A)

PDB ID 3t2x

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