3gjn

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[[Image:3gjn.png|left|200px]]
 
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==Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins==
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The line below this paragraph, containing "STRUCTURE_3gjn", creates the "Structure Box" on the page.
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<StructureSection load='3gjn' size='340' side='right'caption='[[3gjn]], [[Resolution|resolution]] 2.48&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3gjn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GJN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.48&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3gjn| PDB=3gjn | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gjn OCA], [https://pdbe.org/3gjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gjn RCSB], [https://www.ebi.ac.uk/pdbsum/3gjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gjn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMM9_ECOLX IMM9_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/3gjn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gjn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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How do intricate multi-residue features such as protein-protein interfaces evolve? To address this question, we evolved a new colicin-immunity binding interaction. We started with Im9, which inhibits its cognate DNase ColE9 at 10(-14) M affinity, and evolved it toward ColE7, which it inhibits promiscuously (Kd &gt; 10(-8) M). Iterative rounds of random mutagenesis and selection toward higher affinity for ColE7, and selectivity (against ColE9 inhibition), led to an approximately 10(5)-fold increase in affinity and a 10(8)-fold increase in selectivity. Analysis of intermediates along the evolved variants revealed that changes in the binding configuration of the Im protein uncovered a latent set of interactions, thus providing the key to the rapid divergence of new Im7 variants. Overall, protein-protein interfaces seem to share the evolvability features of enzymes, that is, the exploitation of promiscuous interactions and alternative binding configurations via 'generalist' intermediates, and the key role of compensatory stabilizing mutations in facilitating the divergence of new functions.
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===Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins===
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Following evolutionary paths to protein-protein interactions with high affinity and selectivity.,Levin KB, Dym O, Albeck S, Magdassi S, Keeble AH, Kleanthous C, Tawfik DS Nat Struct Mol Biol. 2009 Oct;16(10):1049-55. Epub 2009 Sep 13. PMID:19749752<ref>PMID:19749752</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19749752}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3gjn" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19749752 is the PubMed ID number.
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{{ABSTRACT_PUBMED_19749752}}
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==About this Structure==
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[[3gjn]] is a 4 chain structure of [[Colicin]] and [[Colicin Immunity Protein]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GJN OCA].
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==See Also==
==See Also==
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*[[Colicin|Colicin]]
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*[[Colicin 3D structures|Colicin 3D structures]]
*[[Colicin Immunity Protein|Colicin Immunity Protein]]
*[[Colicin Immunity Protein|Colicin Immunity Protein]]
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*[[Directed evolution|Directed evolution]]
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019749752</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Dym, O.]]
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[[Category: Large Structures]]
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[[Category: Tawfik, D S.]]
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[[Category: Dym O]]
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[[Category: Antibiotic]]
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[[Category: Tawfik DS]]
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[[Category: Antimicrobial]]
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[[Category: Bacteriocin]]
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[[Category: Bacteriocin immunity]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase]]
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[[Category: Metal-binding]]
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[[Category: Nuclease]]
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[[Category: Protein-protein complex]]
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Current revision

Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins

PDB ID 3gjn

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