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4ash

From Proteopedia

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[[Image:4ash.png|left|200px]]
 
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{{STRUCTURE_4ash| PDB=4ash | SCENE= }}
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==Crystal structure of the NS6 protease from murine norovirus 1==
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<StructureSection load='4ash' size='340' side='right'caption='[[4ash]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ash]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ASH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ASH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.578&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ash FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ash OCA], [https://pdbe.org/4ash PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ash RCSB], [https://www.ebi.ac.uk/pdbsum/4ash PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ash ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q80J95_9CALI Q80J95_9CALI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Murine noroviruses have emerged as a valuable tool for investigating the molecular basis of infection and pathogenesis of the closely related human noroviruses, which are the major cause of non-bacterial gastroenteritis. The replication of noroviruses relies on the proteolytic processing of a large polyprotein precursor into six non-structural proteins (NS1-2, NS3, NS4, NS5, NS6(pro), NS7(pol)) by the virally-encoded NS6 protease. We report here the crystal structure of MNV NS6(pro), which has been determined to a resolution of 1.6 A. Adventitiously, the crystal contacts are mediated in part by the binding of the C-terminus of NS6(pro) within the peptide-binding cleft of a neighbouring molecule. This insertion occurs for both molecules in the asymmetric unit of the crystal in a manner that is consistent with physiologically-relevant binding, thereby providing two independent views of a protease-peptide complex. Since the NS6(pro) C-terminus is formed in vivo by NS6(pro) processing, these crystal contacts replicate the protease-product complex that is formed immediately following cleavage of the peptide bond at the NS6-NS7 junction. The observed mode of binding of the C-terminal product peptide yields new insights into the structural basis of NS6(pro) specificity.
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===Crystal structure of the NS6 protease from murine norovirus 1===
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Structure of a Murine Norovirus NS6 Protease-Product Complex Revealed by Adventitious Crystallisation.,Leen EN, Baeza G, Curry S PLoS One. 2012;7(6):e38723. Epub 2012 Jun 7. PMID:22685603<ref>PMID:22685603</ref>
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{{ABSTRACT_PUBMED_22685603}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ash" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[4ash]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ASH OCA].
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*[[Virus protease 3D structures|Virus protease 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022685603</ref><references group="xtra"/>
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__TOC__
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[[Category: Calicivirin]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Murine norovirus 1]]
[[Category: Murine norovirus 1]]
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[[Category: Baeza, G.]]
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[[Category: Baeza G]]
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[[Category: Curry, S.]]
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[[Category: Curry S]]
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[[Category: Leen, E N.]]
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[[Category: Leen EN]]
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[[Category: Calicivirus]]
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[[Category: Hydrolase]]
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[[Category: Trypsin-like]]
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Current revision

Crystal structure of the NS6 protease from murine norovirus 1

PDB ID 4ash

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