2if1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (19:04, 29 May 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2if1.jpg|left|200px]]<br /><applet load="2if1" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2if1" />
 
-
'''HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES'''<br />
 
-
==Overview==
+
==HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES==
-
eIF1 is a universally conserved translation factor that is necessary for, scanning and involved in initiation site selection. We have determined the, solution structure of human eIF1 with an N-terminal His tag using NMR, spectroscopy. Residues 29-113 of the native sequence form a tightly packed, domain with two alpha-helices on one side of a five-stranded parallel and, antiparallel beta-sheet. The fold is new but similar to that of several, ribosomal proteins and RNA-binding domains. A likely binding site is, indicated by yeast mutations and conserved residues located together on, the surface. No interaction with recombinant eIF5 or the initiation site, RNA GCCACAAUGGCA was detected by NMR, but GST pull-down experiments show, that eIF1 binds specifically to the p110 subunit of eIF3. This interaction, explains how eIF1 is recruited to the 40S ribosomal subunit.
+
<StructureSection load='2if1' size='340' side='right'caption='[[2if1]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2if1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IF1 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2if1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2if1 OCA], [https://pdbe.org/2if1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2if1 RCSB], [https://www.ebi.ac.uk/pdbsum/2if1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2if1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/EIF1_HUMAN EIF1_HUMAN] Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/2if1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2if1 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
eIF1 is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. We have determined the solution structure of human eIF1 with an N-terminal His tag using NMR spectroscopy. Residues 29-113 of the native sequence form a tightly packed domain with two alpha-helices on one side of a five-stranded parallel and antiparallel beta-sheet. The fold is new but similar to that of several ribosomal proteins and RNA-binding domains. A likely binding site is indicated by yeast mutations and conserved residues located together on the surface. No interaction with recombinant eIF5 or the initiation site RNA GCCACAAUGGCA was detected by NMR, but GST pull-down experiments show that eIF1 binds specifically to the p110 subunit of eIF3. This interaction explains how eIF1 is recruited to the 40S ribosomal subunit.
-
==About this Structure==
+
Structure and interactions of the translation initiation factor eIF1.,Fletcher CM, Pestova TV, Hellen CU, Wagner G EMBO J. 1999 May 4;18(9):2631-7. PMID:10228174<ref>PMID:10228174</ref>
-
2IF1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IF1 OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure and interactions of the translation initiation factor eIF1., Fletcher CM, Pestova TV, Hellen CU, Wagner G, EMBO J. 1999 May 4;18(9):2631-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10228174 10228174]
+
</div>
-
[[Category: Homo sapiens]]
+
<div class="pdbe-citations 2if1" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Fletcher, C.M.]]
+
-
[[Category: Hellen, C.U.T.]]
+
-
[[Category: Pestova, T.V.]]
+
-
[[Category: Wagner, G.]]
+
-
[[Category: translation initiation factor]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:35:57 2008''
+
==See Also==
 +
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
 +
[[Category: Fletcher CM]]
 +
[[Category: Hellen CUT]]
 +
[[Category: Pestova TV]]
 +
[[Category: Wagner G]]

Current revision

HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES

PDB ID 2if1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2if1"
Personal tools