Pre-mRNA-splicing factor

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{{STRUCTURE_3e9l| PDB=3e9l | SIZE=400| SCENE= |right|CAPTION=Human pre-mRNA-splicing factor 8 endonuclease domain complex with Na+ and Cl- ions, [[3e9l]] }}
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<StructureSection load='' size='350' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>
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__TOC__
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== Function ==
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'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing<ref>PMID:10394358</ref>.
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'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing. For details on pre-mRNA-splicing factors and Prp24 see [[Prp24]]; see detailed [[Prp19]].
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*'''Prp3b''' is part of the U2 small nuclear ribonucleoproteins complex (U2 snRNP)<br />
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*'''Prp8''' functions as a scaffold which mediates the ordered assembly of spliceosomal proteins and snRNAs. For details see [[Prp8]]<br />
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*'''Prp18''' and '''Bud31''' are essential for the catalytic step in pre-mRNA splicing process.<br />
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*'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br />
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*For '''Prp19''' see [[Prp19]]<br />
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*For '''Prp20''' see [[Srp20-Human Alternative Splicing Factor]]<br />
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*'''Prp31''' functions in forming the U4/U5-U6 tri-snRNP.<br />
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*For '''Prp40''' see [[Prp40]].<br />
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*'''Prpcwc2''' is a yeast splicing factor which plays a role in the generation of a functional splicing center '''Prp RBM22''' is the human homolog of Prpcwc2.<br />
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*'''Prpcwc26''' or '''Bud13''' belongs to the pre-mRNA retention and splicing complex RES.<br />
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{{TOC limit|limit=2}}
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== Disease ==
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Mutations in Prp8 and Prp31 cause retinitis pigmentosa<ref>PMID:11468273</ref>.
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== Structural highlights ==
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<scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.
== 3D Structures of pre-mRNA splicing factors ==
== 3D Structures of pre-mRNA splicing factors ==
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[[Pre-mRNA splicing factors 3D structures]]
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''Update June 2012''
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</StructureSection>
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===Pre-mRNA-splicing factor 2===
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[[3beg]] – hPrp residues 105-219 + Ser/Thr-protein kinase – human<BR />
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[[2o3d]] – hPrp P33 subunit
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===Pre-mRNA-splicing factor 3===
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[[1x4q]] – hPrp PWI domain - NMR<BR />
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===Pre-mRNA-splicing factor 3B===
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[[3lqv]], [[2f9d]], [[2f9j]] – hPrp residues 377-415 + pre-mRNA branch site protein 14<BR />
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[[2fho]] - hPrp residues 379-424 + spliceosomal protein P14 - NMR<BR />
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[[2do5]] – hPrp subunit 2 – NMR<BR />
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[[1x5t]] - hPrp subunit 4 – NMR<BR />
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[[1x5u]] - hPrp subunit 4 RNA-binding domain – NMR<BR />
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===Pre-mRNA-splicing factor 8===
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[[3sbt]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 – yeast<BR />
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[[3e66]] – yPrp β-finger domain<BR />
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[[3e9o]], [[3e9p]] - yPrp RNA-binding domain<BR />
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[[2p87]], [[2og4]], [[3sbg]] - yPrp C terminal<BR />
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[[3lru]] – hPrp residues 1831-1990<BR />
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[[3e9l]] – hPrp endonuclease domain<BR />
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[[3enb]] - hPrp core domain IV<BR />
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[[2p8r]] – Prp C terminal (mutant) – ''Caenorhabditis elegans''
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===Pre-mRNA-splicing factor 9===
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[[4dgw]] – yPrp + Prp 21 + Prp 11
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===Pre-mRNA-splicing factor 18===
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[[2dk4]] – hPrp splicing factor motif domain - NMR<BR />
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===Pre-mRNA-splicing factor 19===
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[[3lrv]] – yPrp WD40 domain<BR />
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[[2bay]] – yPrp U-box<BR />
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[[1n87]] - yPrp U-box - NMR<BR />
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===Pre-mRNA-splicing factor 20===
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[[2i2y]] – Prp RNA-binding domain + RNA – ''Streptococcus'' – NMR<BR />
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[[2i38]] - hPrp RNA-binding domain (mutant) – NMR<BR />
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===snRNA-associated-splicing factor 24===
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[[2l9w]] – yPrp C terminal – NMR<BR />
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[[2kh9]] – yPrp residues 115-197 + RNA – NMR<BR />
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[[2go9]] – yPrp RNA-binding domains – NMR<BR />
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[[2ghp]] - yPrp RNA-binding domains
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===Pre-mRNA-splicing factor 43P===
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[[2xau]], [[3kx2 – yPrp + ADP
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===Pre-mRNA-splicing factor RBM22===
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[[2ytc]] – yPrp RNA-binding domain - NMR<BR />
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===Pre-mRNA-splicing factor RDS3===
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[[2k0a]] – yPrp - NMR<BR />
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===Pre-mRNA-splicing factor URN1===
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[[2juc]] – yPrp FF domain - NMR<BR />
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry 3zef)

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References

  1. ↑ Misteli T, Spector DL. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Mol Cell. 1999 Jun;3(6):697-705. PMID:10394358
  2. ↑ McKie AB, McHale JC, Keen TJ, Tarttelin EE, Goliath R, van Lith-Verhoeven JJ, Greenberg J, Ramesar RS, Hoyng CB, Cremers FP, Mackey DA, Bhattacharya SS, Bird AC, Markham AF, Inglehearn CF. Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal dominant retinitis pigmentosa (RP13). Hum Mol Genet. 2001 Jul 15;10(15):1555-62. PMID:11468273

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