2ypi

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[[Image:2ypi.jpg|left|200px]]<br /><applet load="2ypi" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ypi, resolution 2.5&Aring;" />
 
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'''CRYSTALLOGRAPHIC ANALYSIS OF THE COMPLEX BETWEEN TRIOSEPHOSPHATE ISOMERASE AND 2-PHOSPHOGLYCOLATE AT 2.5-ANGSTROMS RESOLUTION. IMPLICATIONS FOR CATALYSIS'''<br />
 
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==Overview==
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==CRYSTALLOGRAPHIC ANALYSIS OF THE COMPLEX BETWEEN TRIOSEPHOSPHATE ISOMERASE AND 2-PHOSPHOGLYCOLATE AT 2.5-ANGSTROMS RESOLUTION. IMPLICATIONS FOR CATALYSIS==
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The binding of the transition-state analogue 2-phosphoglycolate to, triosephosphate isomerase from yeast has been investigated, crystallographically. An atomic model of the enzyme-inhibitor complex has, been refined against data to 2.5-A resolution to a final R factor of 0.18., The interactions between the inhibitor and enzyme have been analyzed. The, inhibitor forms hydrogen bonds to the side chains of His 95 and Glu 165., The latter hydrogen bond confirms that Glu 165 is protonated upon PGA, binding. The structure of the complexed enzyme has been compared to that, of the unbound form of the enzyme, and conformational changes have been, observed: the side chain of Glu 165 moves over 2 A and a 10-residue, flexible loop moves over 7 A to close over the active site. Spectroscopic, results of phosphoglycolic acid binding to triosephosphate isomerase that, have been amassed over the years are also explained in structural terms., The implications for catalysis are noted.
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<StructureSection load='2ypi' size='340' side='right'caption='[[2ypi]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ypi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The February 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''The Glycolytic Enzymes'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_2 10.2210/rcsb_pdb/mom_2004_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YPI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ypi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ypi OCA], [https://pdbe.org/2ypi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ypi RCSB], [https://www.ebi.ac.uk/pdbsum/2ypi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ypi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TPIS_YEAST TPIS_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yp/2ypi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ypi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2YPI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=PGA:'>PGA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 2YPI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb50_1.html The Glycolytic Enzymes]]. Active as [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YPI OCA].
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*[[Triose Phosphate Isomerase|Triose Phosphate Isomerase]]
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*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
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==Reference==
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__TOC__
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Crystallographic analysis of the complex between triosephosphate isomerase and 2-phosphoglycolate at 2.5-A resolution: implications for catalysis., Lolis E, Petsko GA, Biochemistry. 1990 Jul 17;29(28):6619-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2204418 2204418]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
 
[[Category: The Glycolytic Enzymes]]
[[Category: The Glycolytic Enzymes]]
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[[Category: Triose-phosphate isomerase]]
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[[Category: Lolis E]]
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[[Category: Lolis, E.]]
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[[Category: Petsko GA]]
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[[Category: Petsko, G.A.]]
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[[Category: PGA]]
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[[Category: triose phosphate isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:42:44 2008''
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Current revision

CRYSTALLOGRAPHIC ANALYSIS OF THE COMPLEX BETWEEN TRIOSEPHOSPHATE ISOMERASE AND 2-PHOSPHOGLYCOLATE AT 2.5-ANGSTROMS RESOLUTION. IMPLICATIONS FOR CATALYSIS

PDB ID 2ypi

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