2whs

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[[Image:2whs.png|left|200px]]
 
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{{STRUCTURE_2whs| PDB=2whs | SCENE= }}
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==Fluorescent Protein mKeima at pH 3.8==
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<StructureSection load='2whs' size='340' side='right'caption='[[2whs]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2whs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Montipora_sp._20 Montipora sp. 20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WHS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2whs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2whs OCA], [https://pdbe.org/2whs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2whs RCSB], [https://www.ebi.ac.uk/pdbsum/2whs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2whs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q1JV70_9CNID Q1JV70_9CNID]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wh/2whs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2whs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The recently developed red fluorescent protein Keima exhibits the largest Stokes shift (180 nm) observed to date. Combining X-ray crystallography with (in crystallo) UV-visible absorption, fluorescence, and Raman spectroscopy, we have investigated molecular determinants of this peculiar property. The results demonstrate a pH-dependent "reverse chromophore protonation" triggered by the key residue Asp157 and which couples to cis/trans isomerization of the chromophore. These data provided guidelines to rationally design a useful Keima variant.
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===Fluorescent Protein mKeima at pH 3.8===
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Reverse pH-dependence of chromophore protonation explains the large Stokes shift of the red fluorescent protein mKeima.,Violot S, Carpentier P, Blanchoin L, Bourgeois D J Am Chem Soc. 2009 Aug 5;131(30):10356-7. PMID:19722611<ref>PMID:19722611</ref>
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{{ABSTRACT_PUBMED_19722611}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2whs" style="background-color:#fffaf0;"></div>
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[[2whs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Montipora_sp._20 Montipora sp. 20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WHS OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019722611</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Montipora sp. 20]]
[[Category: Montipora sp. 20]]
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[[Category: Blanchoin, L.]]
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[[Category: Blanchoin L]]
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[[Category: Bourgeois, D.]]
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[[Category: Bourgeois D]]
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[[Category: Carpentier, P.]]
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[[Category: Carpentier P]]
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[[Category: Violot, S.]]
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[[Category: Violot S]]
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[[Category: Fluorescent protein]]
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[[Category: Mkeima]]
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[[Category: Stokes shift]]
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Current revision

Fluorescent Protein mKeima at pH 3.8

PDB ID 2whs

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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