4eob

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[[Image:4eob.png|left|200px]]
 
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{{STRUCTURE_4eob| PDB=4eob | SCENE= }}
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==Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa==
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<StructureSection load='4eob' size='340' side='right'caption='[[4eob]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4eob]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EOB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.611&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eob OCA], [https://pdbe.org/4eob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eob RCSB], [https://www.ebi.ac.uk/pdbsum/4eob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eob ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TSE1_PSEAE TSE1_PSEAE] Toxin secreted by the H1 type VI (H1-T6SS) secretion system into the periplasm of recipient cells. Degrades peptidoglycan via amidase activity thereby helping itself to compete with other bacteria (PubMed:21776080, PubMed:22700987, PubMed:22813741, PubMed:22931054). To protect itself, the bacterium synthesizes immunity protein Tsi1 that specifically interacts with and inactivates cognate toxin (PubMed:21776080, PubMed:22700987, PubMed:22931054).<ref>PMID:21776080</ref> <ref>PMID:22700987</ref> <ref>PMID:22813741</ref> <ref>PMID:22931054</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The target range of a bacterial secretion system can be defined by effector substrate specificity or by the efficacy of effector delivery. Here, we report the crystal structure of Tse1, a type VI secretion (T6S) bacteriolytic amidase effector from Pseudomonas aeruginosa. Consistent with its role as a toxin, Tse1 has a more accessible active site than related housekeeping enzymes. The activity of Tse1 against isolated peptidoglycan shows its capacity to act broadly against Gram-negative bacteria and even certain Gram-positive species. Studies with intact cells indicate that Gram-positive bacteria can remain vulnerable to Tse1 despite cell wall modifications. However, interbacterial competition studies demonstrate that Tse1-dependent lysis is restricted to Gram-negative targets. We propose that the previously observed specificity for T6S against Gram-negative bacteria is a consequence of high local effector concentration achieved by T6S-dependent targeting to its site of action rather than inherent effector substrate specificity.
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===Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa===
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Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system.,Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD Cell Rep. 2012 Jun 28;1(6):656-64. Epub 2012 May 31. PMID:22813741<ref>PMID:22813741</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[4eob]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EOB OCA].
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<div class="pdbe-citations 4eob" style="background-color:#fffaf0;"></div>
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[[Category: Pseudomonas aeruginosa]]
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== References ==
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[[Category: Chou, S.]]
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<references/>
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[[Category: Mougous, J D.]]
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__TOC__
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[[Category: Amidase]]
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</StructureSection>
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[[Category: Bacteriolytic]]
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[[Category: Large Structures]]
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[[Category: Hydrolase]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Pa1845]]
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[[Category: Chou S]]
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[[Category: Peptidoglycan degrading]]
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[[Category: Mougous JD]]
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[[Category: Protease]]
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[[Category: Tsi1]]
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Current revision

Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa

PDB ID 4eob

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