2lkd
From Proteopedia
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| - | [[Image:2lkd.png|left|200px]] | ||
| - | + | ==IF2-G2 GDP complex== | |
| + | <StructureSection load='2lkd' size='340' side='right'caption='[[2lkd]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2lkd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKD FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkd OCA], [https://pdbe.org/2lkd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkd RCSB], [https://www.ebi.ac.uk/pdbsum/2lkd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/IF2_GEOSE IF2_GEOSE] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial translation initiation factor IF2 promotes ribosomal subunit association, recruitment, and binding of fMet-tRNA to the ribosomal P-site and initiation dipeptide formation. Here, we present the solution structures of GDP-bound and apo-IF2-G2 of Bacillus stearothermophilus and provide evidence that this isolated domain binds the 50 S ribosomal subunit and hydrolyzes GTP. Differences between the free and GDP-bound structures of IF2-G2 suggest that domain reorganization within the G2-G3-C1 regions underlies the different structural requirements of IF2 during the initiation process. However, these structural signals are unlikely forwarded from IF2-G2 to the C-terminal fMet-tRNA binding domain (IF2-C2) because the connected IF2-C1 and IF2-C2 modules show completely independent mobility, indicating that the bacterial interdomain connector lacks the rigidity that was found in the archaeal IF2 homolog aIF5B. | ||
| - | + | Structural Dynamics of Bacterial Translation Initiation Factor IF2.,Wienk H, Tishchenko E, Belardinelli R, Tomaselli S, Dongre R, Spurio R, Folkers GE, Gualerzi CO, Boelens R J Biol Chem. 2012 Mar 30;287(14):10922-32. Epub 2012 Feb 3. PMID:22308033<ref>PMID:22308033</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2lkd" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
| - | [[Category: Belardinelli | + | [[Category: Large Structures]] |
| - | [[Category: Boelens | + | [[Category: Belardinelli R]] |
| - | [[Category: Dongre | + | [[Category: Boelens R]] |
| - | [[Category: Folkers | + | [[Category: Dongre R]] |
| - | [[Category: Gualerzi | + | [[Category: Folkers GE]] |
| - | [[Category: Spurio | + | [[Category: Gualerzi CO]] |
| - | [[Category: Tishchenko | + | [[Category: Spurio R]] |
| - | [[Category: Tomaselli | + | [[Category: Tishchenko E]] |
| - | [[Category: Wienk | + | [[Category: Tomaselli S]] |
| - | + | [[Category: Wienk H]] | |
Current revision
IF2-G2 GDP complex
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