3upu

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[[Image:3upu.png|left|200px]]
 
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{{STRUCTURE_3upu| PDB=3upu | SCENE= }}
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==Crystal structure of the T4 Phage SF1B Helicase Dda==
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<StructureSection load='3upu' size='340' side='right'caption='[[3upu]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3upu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UPU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.299&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3upu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upu OCA], [https://pdbe.org/3upu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3upu RCSB], [https://www.ebi.ac.uk/pdbsum/3upu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3upu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DDA_BPT4 DDA_BPT4] DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA.<ref>PMID:12411580</ref> <ref>PMID:18314134</ref> <ref>PMID:22504228</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Helicases move on DNA via an ATP binding and hydrolysis mechanism coordinated by well-characterized helicase motifs. However, the translocation along single-stranded DNA (ssDNA) and the strand separation of double-stranded (dsDNA) may be loosely or tightly coupled. Dda is a phage T4 SF1B helicase with sequence homology to the Pif1 family of helicases that tightly couples translocation to strand separation. The crystal structure of the Dda-ssDNA binary complex reveals a domain referred to as the "pin" that was previously thought to remain static during strand separation. The pin contains a conserved phenylalanine that mediates a transient base-stacking interaction that is absolutely required for separation of dsDNA. The pin is secured at its tip by protein-protein interactions through an extended SH3 domain thereby creating a rigid strut. The conserved interface between the pin and the SH3 domain provides the mechanism for tight coupling of translocation to strand separation.
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===Crystal structure of the T4 Phage SF1B Helicase Dda===
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The T4 Phage SF1B Helicase Dda Is Structurally Optimized to Perform DNA Strand Separation.,He X, Byrd AK, Yun MK, Pemble CW 4th, Harrison D, Yeruva L, Dahl C, Kreuzer KN, Raney KD, White SW Structure. 2012 May 30. PMID:22658750<ref>PMID:22658750</ref>
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{{ABSTRACT_PUBMED_22658750}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3upu" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3upu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPU OCA].
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022658750</ref><references group="xtra"/>
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__TOC__
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[[Category: DNA helicase]]
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</StructureSection>
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[[Category: Enterobacteria phage t4]]
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[[Category: Escherichia virus T4]]
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[[Category: He, X.]]
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[[Category: Large Structures]]
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[[Category: IV, C W.Pemble.]]
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[[Category: He X]]
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[[Category: Kreuzer, K N.]]
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[[Category: Kreuzer KN]]
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[[Category: Raney, K D.]]
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[[Category: Pemble IV CW]]
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[[Category: White, S W.]]
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[[Category: Raney KD]]
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[[Category: Yun, M K.]]
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[[Category: White SW]]
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[[Category: Coupling atp hydrolysis to dna unwinding]]
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[[Category: Yun MK]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Pin-tower interface]]
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[[Category: Reca-like domain]]
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[[Category: Sh3 domain]]
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[[Category: Ssdna]]
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Current revision

Crystal structure of the T4 Phage SF1B Helicase Dda

PDB ID 3upu

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