2lsl

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[[Image:2lsl.png|left|200px]]
 
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{{STRUCTURE_2lsl| PDB=2lsl | SCENE= }}
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==Solution structure of the C-terminal domain of Tetrahymena telomerase protein p65==
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<StructureSection load='2lsl' size='340' side='right'caption='[[2lsl]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2lsl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LSL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lsl OCA], [https://pdbe.org/2lsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lsl RCSB], [https://www.ebi.ac.uk/pdbsum/2lsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lsl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LARP7_TETTS LARP7_TETTS] RNA-binding protein required for assembly of the holoenzyme telomerase ribonucleoprotein (RNP) complex (PubMed:15131081, PubMed:15696174, PubMed:16507983, PubMed:17322903, PubMed:20713447, PubMed:22315458, PubMed:22705372). Telomerase is an essential ribonucleoprotein enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:15696174, PubMed:17322903, PubMed:20713447). TAP65/p65 specifically binds telomerase RNA template TER and is required for biogenesis and placement of the TER stem-terminus element: TAP65/p65 first protects the 3'-end of TER from degradation and acts as a chaperone to correctly fold TER for protein binding; it then bends TER stem-loop IV to position it for interaction of stem-loop IV with catalytic TERT RNA-binding domain (PubMed:15696174, PubMed:17322903, PubMed:20713447, PubMed:22315458, PubMed:22705372).<ref>PMID:15131081</ref> <ref>PMID:15696174</ref> <ref>PMID:16507983</ref> <ref>PMID:17322903</ref> <ref>PMID:20713447</ref> <ref>PMID:22315458</ref> <ref>PMID:22705372</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Telomerase is a ribonucleoprotein complex essential for maintenance of telomere DNA at linear chromosome ends. The catalytic core of Tetrahymena telomerase comprises a ternary complex of telomerase RNA (TER), telomerase reverse transcriptase (TERT), and the essential La family protein p65. NMR and crystal structures of p65 C-terminal domain and its complex with stem IV of TER reveal that RNA recognition is achieved by a combination of single- and double-stranded RNA binding, which induces a 105 degrees bend in TER. The domain is a cryptic, atypical RNA recognition motif with a disordered C-terminal extension that forms an alpha helix in the complex necessary for hierarchical assembly of TERT with p65-TER. This work provides the first structural insight into biogenesis and assembly of TER with a telomerase-specific protein. Additionally, our studies define a structurally homologous domain (xRRM) in genuine La and LARP7 proteins and suggest a general mode of RNA binding for biogenesis of their diverse RNA targets.
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===Solution structure of the C-terminal domain of Tetrahymena telomerase protein p65===
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Structural Basis for Telomerase RNA Recognition and RNP Assembly by the Holoenzyme La Family Protein p65.,Singh M, Wang Z, Koo BK, Patel A, Cascio D, Collins K, Feigon J Mol Cell. 2012 Jun 14. PMID:22705372<ref>PMID:22705372</ref>
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{{ABSTRACT_PUBMED_22705372}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2lsl" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2lsl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LSL OCA].
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*[[Telomerase-associated protein|Telomerase-associated protein]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022705372</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
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[[Category: Cascio, D.]]
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[[Category: Cascio D]]
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[[Category: Collins, K.]]
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[[Category: Collins K]]
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[[Category: Feigon, J.]]
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[[Category: Feigon J]]
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[[Category: Koo, B.]]
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[[Category: Koo B]]
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[[Category: Patel, A.]]
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[[Category: Patel A]]
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[[Category: Singh, M.]]
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[[Category: Singh M]]
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[[Category: Wang, Z.]]
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[[Category: Wang Z]]
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[[Category: La protein]]
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[[Category: Larp7]]
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[[Category: P65]]
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[[Category: Rna binding protein]]
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[[Category: Rrm]]
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[[Category: Telomerase]]
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[[Category: Tetrahymena]]
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Current revision

Solution structure of the C-terminal domain of Tetrahymena telomerase protein p65

PDB ID 2lsl

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