3vmv

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[[Image:3vmv.jpg|left|200px]]
 
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{{STRUCTURE_3vmv| PDB=3vmv | SCENE= }}
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==Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165==
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<StructureSection load='3vmv' size='340' side='right'caption='[[3vmv]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3vmv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._N16-5 Bacillus sp. N16-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VMV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vmv OCA], [https://pdbe.org/3vmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vmv RCSB], [https://www.ebi.ac.uk/pdbsum/3vmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vmv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D0VP31_9BACI D0VP31_9BACI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The pectate lyase (Bsp165PelA) from Bacillus sp. N16-5 has great potential in industrial applications because it shows high specific activity under extremely alkaline conditions. Besides, activity measurement of Bsp165PelA does not require addition of calcium, in a way different from the other pectate lyases. Here we report crystal structures of Bsp165PelA in apo-form and in complex with trigalacturonate. The parallel beta-helix, active site residues and substrate binding cleft are similar to those in the other pectate lyases from Polysaccharide Lyase family 1. However, some of the highly conserved Ca(2+) binding residues and secondary structures are altered in Bsp165PelA, making it difficult to coordinate with Ca(2+) as in the other pectate lyases. We found Bsp165PelA forms some direct enzyme-substrate interactions instead of using Ca(2+) ions bridging in the extremely alkaline environment.
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===Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165===
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Crystal structure and substrate-binding mode of a novel pectate lyase from alkaliphilic Bacillus sp. N16-5.,Zheng Y, Huang CH, Liu W, Ko TP, Xue Y, Zhou C, Guo RT, Ma Y Biochem Biophys Res Commun. 2012 Apr 6;420(2):269-74. Epub 2012 Mar 3. PMID:22414692<ref>PMID:22414692</ref>
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{{ABSTRACT_PUBMED_22414692}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3vmv" style="background-color:#fffaf0;"></div>
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[[3vmv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus Bacillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VMV OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:022414692</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Bacillus]]
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[[Category: Bacillus sp. N16-5]]
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[[Category: Pectate lyase]]
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[[Category: Large Structures]]
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[[Category: Guo, R T.]]
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[[Category: Guo RT]]
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[[Category: Huang, C H.]]
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[[Category: Huang CH]]
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[[Category: Ko, T P.]]
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[[Category: Ko TP]]
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[[Category: Liu, W.]]
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[[Category: Liu W]]
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[[Category: Ma, Y.]]
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[[Category: Ma Y]]
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[[Category: Xue, Y.]]
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[[Category: Xue Y]]
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[[Category: Zhang, G.]]
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[[Category: Zhang G]]
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[[Category: Zheng, Y.]]
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[[Category: Zheng Y]]
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[[Category: Zhou, C.]]
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[[Category: Zhou C]]
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[[Category: Beta-helix]]
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[[Category: Lyase]]
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[[Category: Pectolytic]]
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[[Category: Polygalacturonate]]
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[[Category: Polysaccharide lyase family 1]]
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Current revision

Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165

PDB ID 3vmv

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