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1aa6

From Proteopedia

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[[Image:1aa6.gif|left|200px]]<br /><applet load="1aa6" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1aa6, resolution 2.3&Aring;" />
 
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'''REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''<br />
 
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==Overview==
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==REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI==
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Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.
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<StructureSection load='1aa6' size='340' side='right'caption='[[1aa6]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1aa6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AA6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aa6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aa6 OCA], [https://pdbe.org/1aa6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aa6 RCSB], [https://www.ebi.ac.uk/pdbsum/1aa6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aa6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FDHF_ECOLI FDHF_ECOLI] Decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/1aa6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aa6 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=MGD:'>MGD</scene> and <scene name='pdbligand=4MO:'>4MO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Known structural/functional Sites: <scene name='pdbsite=4MO:The+Mo+Atom+Is+Coordinated+To+The+Se+Atom+Of+Sec+140+And+...'>4MO</scene> and <scene name='pdbsite=FS4:The+Fe+S+Cluster+Is+Coordinated+To+The+S+Atoms+Of+CYS+8,+...'>FS4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA].
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*[[Formate dehydrogenase 3D structures|Formate dehydrogenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9036855 9036855]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Formate dehydrogenase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Boyington JC]]
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[[Category: Boyington, J C.]]
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[[Category: Sun PD]]
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[[Category: Sun, P D.]]
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[[Category: 4MO]]
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[[Category: MGD]]
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[[Category: SF4]]
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[[Category: anaerobic]]
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[[Category: dehydrogenase]]
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[[Category: fe4s4]]
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[[Category: formate]]
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[[Category: iron sulfur cluster]]
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[[Category: mgd]]
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[[Category: molybdenum]]
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[[Category: molybdopterin]]
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[[Category: molybdopterin guanine dinucleotide]]
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[[Category: mpt]]
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[[Category: oxidoreductase]]
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[[Category: secys]]
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[[Category: selenium]]
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[[Category: selenocysteine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:33 2008''
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Current revision

REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI

PDB ID 1aa6

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