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1aif

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[[Image:1aif.gif|left|200px]]<br /><applet load="1aif" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1aif, resolution 2.9&Aring;" />
 
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'''ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE'''<br />
 
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==Overview==
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==ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE==
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The crystal structure of anti-idiotopic Fab 409.5.3, made against an E2 specific feline infectious peritonitis virus-neutralizing antibody 730.1.4, has been determined in its free from, at 2.9 A resolution by molecular replacement. This antibody, used as an immmunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The structure of the uncomplexed Fab was refined using constrained-restrained least squares minimization and simulated annealing in combination with conjugate gradient techniques to a crystallographic R of 0.22 based on 16,482 unique reflections between 20.0 and 2.9 A. The free antiidiotypic Fab shows, when compared to its complexed form, a 5 degrees rotation of its variable light with respect to its variable heavy domain and rearrangement of complementarity determining region loops, which permits optimization of the stereocomplementarity between interacting molecules. This finding supports the induced fit hypothesis for antibody antigen interaction.
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<StructureSection load='1aif' size='340' side='right'caption='[[1aif]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1aif]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AIF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aif OCA], [https://pdbe.org/1aif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aif RCSB], [https://www.ebi.ac.uk/pdbsum/1aif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aif ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1aif_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aif ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AIF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIF OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Structure of an anti-idiotypic Fab against feline peritonitis virus-neutralizing antibody and a comparison with the complexed Fab., Ban N, Escobar C, Hasel KW, Day J, Greenwood A, McPherson A, FASEB J. 1995 Jan;9(1):107-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7821749 7821749]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Ban N]]
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[[Category: Ban, N.]]
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[[Category: Day J]]
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[[Category: Day, J.]]
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[[Category: Escobar C]]
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[[Category: Escobar, C.]]
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[[Category: Greenwood A]]
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[[Category: Greenwood, A.]]
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[[Category: Hasel K]]
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[[Category: Hasel, K.]]
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[[Category: McPherson A]]
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[[Category: McPherson, A.]]
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[[Category: c region]]
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[[Category: immunoglobulin]]
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[[Category: v region]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:48 2008''
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Current revision

ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE

PDB ID 1aif

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