3eq3

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[[Image:3eq3.png|left|200px]]
 
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{{STRUCTURE_3eq3| PDB=3eq3 | SCENE= }}
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==Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM==
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<SX load='3eq3' size='340' side='right' viewer='molstar' caption='[[3eq3]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
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===Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3eq3]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EQ3 FirstGlance]. <br>
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{{ABSTRACT_PUBMED_19020518}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eq3 OCA], [https://pdbe.org/3eq3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eq3 RCSB], [https://www.ebi.ac.uk/pdbsum/3eq3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eq3 ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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[[3eq3]] is a 9 chain structure of [[Ribosomal protein L11]] and [[Ribosomal protein S12]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQ3 OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/RS12_ECOLI RS12_ECOLI] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_00403_B] Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.[HAMAP-Rule:MF_00403_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eq/3eq3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eq3 ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
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*[[Ribosomal protein L11|Ribosomal protein L11]]
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019020518</ref><references group="xtra"/>
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</SX>
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[[Category: Escherichia coli k12]]
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[[Category: Escherichia coli K-12]]
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[[Category: Agirrezabala, X.]]
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[[Category: Large Structures]]
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[[Category: Frank, J.]]
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[[Category: Agirrezabala X]]
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[[Category: Li, W.]]
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[[Category: Frank J]]
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[[Category: A/t-trna]]
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[[Category: Li W]]
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[[Category: Antibiotic resistance]]
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[[Category: Automated data collection]]
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[[Category: Elongation factor]]
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[[Category: Gtp-binding]]
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[[Category: Membrane]]
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[[Category: Methylation]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Protein biosynthesis]]
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[[Category: Protein translation]]
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[[Category: Ribonucleoprotein]]
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[[Category: Ribosomal protein]]
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[[Category: Ribosomal protein-rna complex]]
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[[Category: Rna-binding]]
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[[Category: Rrna-binding]]
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[[Category: Ternary complex]]
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[[Category: Trna-binding]]
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Current revision

Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM

3eq3, resolution 9.00Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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