1amx

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[[Image:1amx.jpg|left|200px]]<br /><applet load="1amx" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1amx, resolution 2.0&Aring;" />
 
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'''COLLAGEN-BINDING DOMAIN FROM A STAPHYLOCOCCUS AUREUS ADHESIN'''<br />
 
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==Overview==
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==COLLAGEN-BINDING DOMAIN FROM A STAPHYLOCOCCUS AUREUS ADHESIN==
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The crystal structure of the recombinant 19,000 M(r) binding domain from the Staphylococcus aureus collagen adhesin has been determined at 2 A resolution. The domain fold is a jelly-roll, composed of two antiparallel beta-sheets and two short alpha-helices. Triple-helical collagen model probes were used in a systematic docking search to identify the collagen-binding site. A groove on beta-sheet I exhibited the best surface complementarity to the collagen probes. This site partially overlaps with the peptide sequence previously shown to be critical for collagen binding. Recombinant proteins containing single amino acid mutations designed to disrupt the surface of the putative binding site exhibited significantly lower affinities for collagen. Here we present a structural perspective for the mode of collagen binding by a bacterial surface protein.
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<StructureSection load='1amx' size='340' side='right'caption='[[1amx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1amx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AMX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1amx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1amx OCA], [https://pdbe.org/1amx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1amx RCSB], [https://www.ebi.ac.uk/pdbsum/1amx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1amx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CNA_STAAU CNA_STAAU] Mediates attachment of staphylococcal cells to collagen-containing substrata.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/am/1amx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1amx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AMX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Known structural/functional Site: <scene name='pdbsite=CBR:Collagen-Binding+Residues+Identified+By+Site-Directed+Mu+...'>CBR</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AMX OCA].
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*[[Adhesin 3D structures|Adhesin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the collagen-binding domain from a Staphylococcus aureus adhesin., Symersky J, Patti JM, Carson M, House-Pompeo K, Teale M, Moore D, Jin L, Schneider A, DeLucas LJ, Hook M, Narayana SV, Nat Struct Biol. 1997 Oct;4(10):833-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9334749 9334749]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Narayana, S.]]
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[[Category: Narayana S]]
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[[Category: Symersky, J.]]
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[[Category: Symersky J]]
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[[Category: bacterial adhesin]]
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[[Category: mscramm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:46:16 2008''
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Current revision

COLLAGEN-BINDING DOMAIN FROM A STAPHYLOCOCCUS AUREUS ADHESIN

PDB ID 1amx

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