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1au1

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[[Image:1au1.gif|left|200px]]<br /><applet load="1au1" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1au1, resolution 2.2&Aring;" />
 
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'''HUMAN INTERFERON-BETA CRYSTAL STRUCTURE'''<br />
 
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==Overview==
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==HUMAN INTERFERON-BETA CRYSTAL STRUCTURE==
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Type I interferons (IFNs) are helical cytokines that have diverse biological activities despite the fact that they appear to interact with the same receptor system. To achieve a better understanding of the structural basis for the different activities of alpha and beta IFNs, we have determined the crystal structure of glycosylated human IFN-beta at 2.2-A resolution by molecular replacement. The molecule adopts a fold similar to that of the previously determined structures of murine IFN-beta and human IFN-alpha2b but displays several distinct structural features. Like human IFN-alpha2b, human IFN-beta contains a zinc-binding site at the interface of the two molecules in the asymmetric unit, raising the question of functional relevance for IFN-beta dimers. However, unlike the human IFN-alpha2b dimer, in which homologous surfaces form the interface, human IFN-beta dimerizes with contact surfaces from opposite sides of the molecule. The relevance of the structure to the effects of point mutations in IFN-beta at specific exposed residues is discussed. A potential role of ligand-ligand interactions in the conformational assembly of IFN receptor components is discussed.
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<StructureSection load='1au1' size='340' side='right'caption='[[1au1]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1au1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The August 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Interferons'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_8 10.2210/rcsb_pdb/mom_2010_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AU1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=G6D:6-DEOXY-ALPHA-D-GLUCOSE'>G6D</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1au1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1au1 OCA], [https://pdbe.org/1au1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1au1 RCSB], [https://www.ebi.ac.uk/pdbsum/1au1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1au1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IFNB_HUMAN IFNB_HUMAN] Has antiviral, antibacterial and anticancer activities.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1au1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1au1 ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: Kaposi sarcoma, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147620 147620]], Osteopenia/osteoporosis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147620 147620]]
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*[[Interferon 3D structures|Interferon 3D structures]]
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*[[Multiple sclerosis|Multiple sclerosis]]
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==About this Structure==
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__TOC__
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1AU1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AU1 OCA].
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</StructureSection>
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==Reference==
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The crystal structure of human interferon beta at 2.2-A resolution., Karpusas M, Nolte M, Benton CB, Meier W, Lipscomb WN, Goelz S, Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11813-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9342320 9342320]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Interferons]]
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[[Category: Karpusas, M.]]
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[[Category: Large Structures]]
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[[Category: Lipscomb, W.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Nolte, M.]]
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[[Category: Karpusas M]]
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[[Category: ZN]]
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[[Category: Lipscomb W]]
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[[Category: cytokine]]
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[[Category: Nolte M]]
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[[Category: helical cytokine]]
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[[Category: immune system]]
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[[Category: interferon]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:17 2008''
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Current revision

HUMAN INTERFERON-BETA CRYSTAL STRUCTURE

PDB ID 1au1

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