2wq8

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[[Image:2wq8.png|left|200px]]
 
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{{STRUCTURE_2wq8| PDB=2wq8 | SCENE= }}
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==Glycan labelling using engineered variants of galactose oxidase obtained by directed evolution==
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<StructureSection load='2wq8' size='340' side='right'caption='[[2wq8]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wq8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_graminearum Fusarium graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WQ8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wq8 OCA], [https://pdbe.org/2wq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wq8 RCSB], [https://www.ebi.ac.uk/pdbsum/2wq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wq8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAOA_GIBZA GAOA_GIBZA] Catalyzes the sterospecific oxidation of primary alcohols to the corresponding aldehydes. The biologically relevant substrate of the enzyme is not known as the enzyme exhibits broad substrate specificity from small alcohols through sugars to oligo- and polysaccharides.<ref>PMID:13641238</ref> <ref>PMID:4441089</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/2wq8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wq8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans d-mannose (Man) and d-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.
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===GLYCAN LABELLING USING ENGINEERED VARIANTS OF GALACTOSE OXIDASE OBTAINED BY DIRECTED EVOLUTION===
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Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution.,Rannes JB, Ioannou A, Willies SC, Grogan G, Behrens C, Flitsch SL, Turner NJ J Am Chem Soc. 2011 Jun 8;133(22):8436-9. Epub 2011 May 12. PMID:21526835<ref>PMID:21526835</ref>
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{{ABSTRACT_PUBMED_21526835}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2wq8" style="background-color:#fffaf0;"></div>
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[[2wq8]] is a 1 chain structure of [[Galactose oxidase]] with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQ8 OCA].
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==See Also==
==See Also==
*[[Galactose oxidase|Galactose oxidase]]
*[[Galactose oxidase|Galactose oxidase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021526835</ref><references group="xtra"/>
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__TOC__
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[[Category: Galactose oxidase]]
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</StructureSection>
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[[Category: Gibberella zeae]]
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[[Category: Fusarium graminearum]]
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[[Category: Behrens, C.]]
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[[Category: Large Structures]]
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[[Category: Flitsch, S L.]]
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[[Category: Behrens C]]
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[[Category: Grogan, G J.]]
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[[Category: Flitsch SL]]
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[[Category: Ioannou, A.]]
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[[Category: Grogan GJ]]
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[[Category: Rannes, J G.]]
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[[Category: Ioannou A]]
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[[Category: Turner, N J.]]
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[[Category: Rannes JG]]
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[[Category: Willies, S C.]]
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[[Category: Turner NJ]]
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[[Category: Directed evolution]]
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[[Category: Willies SC]]
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[[Category: Glycoengineering]]
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[[Category: Kelch repeat]]
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[[Category: Metal-binding]]
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[[Category: Oxidoreductase]]
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Current revision

Glycan labelling using engineered variants of galactose oxidase obtained by directed evolution

PDB ID 2wq8

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