1ayl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:34, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ayl.gif|left|200px]]<br /><applet load="1ayl" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ayl, resolution 1.8&Aring;" />
 
-
'''PHOSPHOENOLPYRUVATE CARBOXYKINASE'''<br />
 
-
==Overview==
+
==PHOSPHOENOLPYRUVATE CARBOXYKINASE==
-
We report the 1.8 A crystal structure of adenosine triphosphate (ATP)-magnesium-oxalate bound phosphoenolpyruvate carboxykinase (PCK) from Escherichia coli. ATP binding induces a 20 degree hinge-like rotation of the N- and C-terminal domains which closes the active-site cleft. PCK possesses a novel nucleotide-binding fold, particularly in the adenine-binding region, where the formation of a cis backbone torsion angle in a loop glycine residue promotes intimate contacts between the adenine-binding loop and adenine, while stabilizing a syn conformation of the base. This complex represents a reaction intermediate analogue along the pathway of the conversion of oxaloacetate to phosphoenolpyruvate, and provides insight into the mechanistic details of the chemical reaction catalysed by this enzyme.
+
<StructureSection load='1ayl' size='340' side='right'caption='[[1ayl]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ayl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AYL FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ayl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayl OCA], [https://pdbe.org/1ayl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ayl RCSB], [https://www.ebi.ac.uk/pdbsum/1ayl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayl ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PCKA_ECOLI PCKA_ECOLI]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/1ayl_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ayl ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1AYL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=OXL:'>OXL</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(ATP) Phosphoenolpyruvate carboxykinase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.49 4.1.1.49] Known structural/functional Site: <scene name='pdbsite=ACT:Putative+Active+Site+Residues'>ACT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYL OCA].
+
*[[Phosphoenolpyruvate carboxykinase 3D structures|Phosphoenolpyruvate carboxykinase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Snapshot of an enzyme reaction intermediate in the structure of the ATP-Mg2+-oxalate ternary complex of Escherichia coli PEP carboxykinase., Tari LW, Matte A, Pugazhenthi U, Goldie H, Delbaere LT, Nat Struct Biol. 1996 Apr;3(4):355-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8599762 8599762]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Escherichia coli]]
+
[[Category: Large Structures]]
-
[[Category: Phosphoenolpyruvate carboxykinase (ATP)]]
+
[[Category: Delbaere LTJ]]
-
[[Category: Single protein]]
+
[[Category: Goldie H]]
-
[[Category: Delbaere, L T.J.]]
+
[[Category: Pugazenthi U]]
-
[[Category: Goldie, H.]]
+
[[Category: Tari LW]]
-
[[Category: Pugazenthi, U.]]
+
-
[[Category: Tari, L W.]]
+
-
[[Category: ATP]]
+
-
[[Category: MG]]
+
-
[[Category: OXL]]
+
-
[[Category: kinase (transphosphorylating)]]
+
-
[[Category: nucleotide-triphosphate hydrolase]]
+
-
[[Category: p-loop]]
+
-
[[Category: protein-atp complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:49:38 2008''
+

Current revision

PHOSPHOENOLPYRUVATE CARBOXYKINASE

PDB ID 1ayl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ayl"
Personal tools