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1azo

From Proteopedia

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[[Image:1azo.jpg|left|200px]]<br /><applet load="1azo" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1azo, resolution 1.70&Aring;" />
 
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'''DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI'''<br />
 
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==Overview==
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==DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI==
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MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli. MutH cleaves a newly synthesized and unmethylated daughter strand 5' to the sequence d(GATC) in a hemi-methylated duplex. Activation of MutH requires the recognition of a DNA mismatch by MutS and MutL. We have crystallized MutH in two space groups and solved the structures at 1.7 and 2.3 A resolution, respectively. The active site of MutH is located at an interface between two subdomains that pivot relative to one another, as revealed by comparison of the crystal structures, and this presumably regulates the nuclease activity. The relative motion of the two subdomains in MutH correlates with the position of a protruding C-terminal helix. This helix appears to act as a molecular lever through which MutS and MutL may communicate the detection of a DNA mismatch and activate MutH. With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor.
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<StructureSection load='1azo' size='340' side='right'caption='[[1azo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1azo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AZO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1azo OCA], [https://pdbe.org/1azo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1azo RCSB], [https://www.ebi.ac.uk/pdbsum/1azo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1azo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTH_ECOLI MUTH_ECOLI] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.[HAMAP-Rule:MF_00759]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/1azo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1azo ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AZO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZO OCA].
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases., Ban C, Yang W, EMBO J. 1998 Mar 2;17(5):1526-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9482749 9482749]
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[[Category: Escherichia coli K-12]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Yang W]]
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[[Category: Yang, W.]]
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[[Category: EDO]]
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[[Category: dna repair]]
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[[Category: endonuclease]]
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[[Category: hydrolase]]
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[[Category: muth]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:50:00 2008''
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Current revision

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

PDB ID 1azo

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