1sks

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[[Image:1sks.png|left|200px]]
 
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{{STRUCTURE_1sks| PDB=1sks | SCENE= }}
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==Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template==
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<StructureSection load='1sks' size='340' side='right'caption='[[1sks]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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===Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sks]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SKS FirstGlance]. <br>
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{{ABSTRACT_PUBMED_15235589}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DT:3-DEOXYTHYMIDINE-5-MONOPHOSPHATE'>2DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTD:CIS-SYN+CYCLOBUTANE+THYMINE+DIMER'>TTD</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sks OCA], [https://pdbe.org/1sks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sks RCSB], [https://www.ebi.ac.uk/pdbsum/1sks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sks ProSAT]</span></td></tr>
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[[1sks]] is a 4 chain structure of [[DNA polymerase]] and [[Thioredoxin]] with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKS OCA].
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPOL_BPT7 DPOL_BPT7] Replicates viral genomic DNA. Non-processive DNA polymerase that achieves processivity by binding to host thioredoxin (TrxA). This interaction increases the rate of dNTP incorporation to yield a processivity of approximately 800 nucleotides (nt) per binding event. Interacts with DNA helicase gp4 to coordinate nucleotide polymerization with unwinding of the DNA. The leading strand is synthesized continuously while synthesis of the lagging strand requires the synthesis of oligoribonucleotides by the primase domain of gp4.<ref>PMID:9218486</ref> <ref>PMID:21606333</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sk/1sks_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sks ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
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*[[DNA polymerase|DNA polymerase]]
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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*[[Thioredoxin|Thioredoxin]]
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*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015235589</ref><references group="xtra"/>
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__TOC__
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[[Category: DNA-directed DNA polymerase]]
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</StructureSection>
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[[Category: Enterobacteria phage t7]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bdour, H M.]]
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[[Category: Escherichia phage T7]]
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[[Category: Doublie, S.]]
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[[Category: Large Structures]]
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[[Category: Dutta, S.]]
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[[Category: Bdour HM]]
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[[Category: Ellenberger, T.]]
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[[Category: Doublie S]]
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[[Category: Li, Y.]]
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[[Category: Dutta S]]
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[[Category: Taylor, J S.]]
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[[Category: Ellenberger T]]
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[[Category: Close]]
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[[Category: Li Y]]
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[[Category: Dna polymerase]]
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[[Category: Taylor JS]]
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[[Category: Fidelity]]
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[[Category: Lesion bypass]]
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[[Category: Open]]
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[[Category: Transferase-electron transport-dna complex]]
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Current revision

Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template

PDB ID 1sks

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