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1bet

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[[Image:1bet.gif|left|200px]]<br /><applet load="1bet" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bet, resolution 2.3&Aring;" />
 
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'''NEW PROTEIN FOLD REVEALED BY A 2.3 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF NERVE GROWTH FACTOR'''<br />
 
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==Overview==
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==NEW PROTEIN FOLD REVEALED BY A 2.3 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF NERVE GROWTH FACTOR==
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Nerve growth factor (NGF) is a member of an expanding family of neurotrophic factors (including brain-derived neurotrophic factor and the neurotrophins) that control the development and survival of certain neuronal populations both in the peripheral and in the central nervous systems. Its biological effects are mediated by a high-affinity ligand-receptor interaction and a tyrosine kinase signalling pathway. A potential use for NGF and its relatives in the treatment of neurological disorders such as Alzheimer's disease and Parkinson's disease requires an understanding of the structure-function relationships of NGF. NGF is a dimeric molecule, with 118 amino acids per protomer. We report the crystal structure of the murine NGF dimer at 2.3-A resolution, which reveals a novel protomer structure consisting of three antiparallel pairs of beta strands, together forming a flat surface. Two subunits associate through this surface, thus burying a total of 2,332 A. Four loop regions, which contain many of the variable residues observed between different NGF-related molecules, may determine the different receptor specificities. A clustering of positively charged side chains may provide a complementary interaction with the acidic low-affinity NGF receptor. The structure provides a model for rational design of analogues of NGF and its relatives and for testing the NGF-receptor recognition determinants critical for signal transduction.
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<StructureSection load='1bet' size='340' side='right'caption='[[1bet]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The August 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Neurotrophins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_8 10.2210/rcsb_pdb/mom_2005_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BET FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bet OCA], [https://pdbe.org/1bet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bet RCSB], [https://www.ebi.ac.uk/pdbsum/1bet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bet ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NGF_MOUSE NGF_MOUSE] Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/1bet_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bet ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BET is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The following page contains interesting information on the relation of 1BET with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb68_1.html Neurotrophins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BET OCA].
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*[[Nerve growth factor|Nerve growth factor]]
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__TOC__
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==Reference==
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</StructureSection>
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New protein fold revealed by a 2.3-A resolution crystal structure of nerve growth factor., McDonald NQ, Lapatto R, Murray-Rust J, Gunning J, Wlodawer A, Blundell TL, Nature. 1991 Dec 5;354(6352):411-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1956407 1956407]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Neurotrophins]]
[[Category: Neurotrophins]]
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[[Category: Single protein]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Blundell, T L.]]
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[[Category: Blundell TL]]
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[[Category: Gunning, J.]]
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[[Category: Gunning J]]
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[[Category: Lapatto, R.]]
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[[Category: Lapatto R]]
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[[Category: Mcdonald, N Q.]]
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[[Category: Mcdonald NQ]]
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[[Category: Murray-Rust, J.]]
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[[Category: Murray-Rust J]]
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[[Category: Wlodawer, A.]]
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[[Category: Wlodawer A]]
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[[Category: growth factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:32 2008''
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Current revision

NEW PROTEIN FOLD REVEALED BY A 2.3 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF NERVE GROWTH FACTOR

PDB ID 1bet

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