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1jbd
From Proteopedia
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| - | [[Image:1jbd.png|left|200px]] | ||
| - | + | ==NMR Structure of the Complex Between alpha-bungarotoxin and a Mimotope of the Nicotinic Acetylcholine Receptor== | |
| - | + | <StructureSection load='1jbd' size='340' side='right'caption='[[1jbd]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1jbd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hn7 1hn7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JBD FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbd OCA], [https://pdbe.org/1jbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jbd RCSB], [https://www.ebi.ac.uk/pdbsum/1jbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jbd ProSAT]</span></td></tr> | |
| - | == | + | </table> |
| - | [[1jbd]] is a 2 chain structure | + | == Function == |
| + | [https://www.uniprot.org/uniprot/3L21A_BUNMU 3L21A_BUNMU] Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=0.4 nM) and neuronal (tested on chimeric alpha-7/CHRNA7, Kd=0.95 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). It also shows an activity on GABA(A) receptors (PubMed:16549768, PubMed:25634239). It antagonises GABA-activated currents with high potency when tested on primary hippocampal neurons (PubMed:25634239). It inhibits recombinantly expressed GABA(A) receptors composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits with high potency (62.3% inhibition at 20 uM of toxin) (PubMed:25634239). It also shows a weaker inhibition on GABA(A) receptors composed of alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) subunits, alpha-4-beta-2-gamma-2 (GABRA4-GABRB2-GABRG2) subunits, and alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:25634239). A very weak inhibition is also observed on GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) (PubMed:26221036). It has also been shown to bind and inhibit recombinant GABA(A) receptor beta-3/GABRB3 subunit (Kd=about 50 nM) (PubMed:16549768). In addition, it blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM) (PubMed:9840221).<ref>PMID:16549768</ref> <ref>PMID:25634239</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/1jbd_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jbd ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Bungarotoxin|Bungarotoxin]] | + | *[[Bungarotoxin 3D structures|Bungarotoxin 3D structures]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Bungarus multicinctus]] | [[Category: Bungarus multicinctus]] | ||
| - | [[Category: Bernini | + | [[Category: Large Structures]] |
| - | [[Category: Bracci | + | [[Category: Bernini A]] |
| - | [[Category: Calamandrei | + | [[Category: Bracci L]] |
| - | [[Category: Ciutti | + | [[Category: Calamandrei D]] |
| - | [[Category: | + | [[Category: Ciutti A]] |
| - | [[Category: | + | [[Category: Di Maro D]] |
| - | [[Category: | + | [[Category: Klein S]] |
| - | [[Category: | + | [[Category: Lelli B]] |
| - | [[Category: Niccolai | + | [[Category: Lozzi L]] |
| - | [[Category: Scarselli | + | [[Category: Niccolai N]] |
| - | [[Category: Spiga | + | [[Category: Scarselli M]] |
| - | + | [[Category: Spiga O]] | |
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Current revision
NMR Structure of the Complex Between alpha-bungarotoxin and a Mimotope of the Nicotinic Acetylcholine Receptor
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Categories: Bungarus multicinctus | Large Structures | Bernini A | Bracci L | Calamandrei D | Ciutti A | Di Maro D | Klein S | Lelli B | Lozzi L | Niccolai N | Scarselli M | Spiga O
