1bk6

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[[Image:1bk6.gif|left|200px]]<br /><applet load="1bk6" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bk6, resolution 2.8&Aring;" />
 
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'''KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS'''<br />
 
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==Overview==
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==KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS==
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Selective nuclear import is mediated by nuclear localization signals (NLSs) and cognate transport factors known as karyopherins or importins. Karyopherin alpha recognizes "classical" monopartite and bipartite NLSs. We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast karyopherin alpha, in the absence and presence of a monopartite NLS peptide at 2.2 A and 2.8 A resolution, respectively. The structure shows a tandem array of ten armadillo repeats, organized in a right-handed superhelix of helices. Binding of the NLS peptide occurs at two sites within a helical surface groove that is lined by conserved residues. The structure reveals the determinants of NLS specificity and suggests a model for the recognition of bipartite NLSs.
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<StructureSection load='1bk6' size='340' side='right'caption='[[1bk6]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bk6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BK6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bk6 OCA], [https://pdbe.org/1bk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bk6 RCSB], [https://www.ebi.ac.uk/pdbsum/1bk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bk6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMA1_YEAST IMA1_YEAST] Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).<ref>PMID:7565597</ref> <ref>PMID:10913188</ref> <ref>PMID:21075847</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/1bk6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bk6 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BK6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BK6 OCA].
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*[[Importin 3D structures|Importin 3D structures]]
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*[[Large T Antigen|Large T Antigen]]
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==Reference==
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== References ==
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Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha., Conti E, Uy M, Leighton L, Blobel G, Kuriyan J, Cell. 1998 Jul 24;94(2):193-204. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9695948 9695948]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Blobel G]]
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[[Category: Blobel, G.]]
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[[Category: Conti E]]
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[[Category: Conti, E.]]
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[[Category: Kuriyan J]]
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[[Category: Kuriyan, J.]]
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[[Category: Leighton L]]
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[[Category: Leighton, L.]]
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[[Category: Uy M]]
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[[Category: Uy, M.]]
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[[Category: armadillo repeat containing protein]]
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[[Category: complex (protein transport/peptide)]]
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[[Category: nls nuclear import]]
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[[Category: protein transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:10 2008''
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Current revision

KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS

PDB ID 1bk6

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