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1bm8

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[[Image:1bm8.jpg|left|200px]]<br /><applet load="1bm8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bm8, resolution 1.71&Aring;" />
 
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'''DNA-BINDING DOMAIN OF MBP1'''<br />
 
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==Overview==
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==DNA-BINDING DOMAIN OF MBP1==
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BACKGROUND: During the cell cycle, cells progress through four distinct phases, G1, S, G2 and M; transcriptional controls play an important role at the transition between these phases. MCB-binding factor (MBF), a transcription factor from budding yeast, binds to the so-called MCB (MluI cell-cycle box) elements found in the promoters of many DNA synthesis genes, and activates the transcription of those at the G1--&gt;S phase transition. MBF is comprised of two proteins, Mbp1 and Swi6. RESULTS: The three-dimensional structure of the N-terminal DNA-binding domain of Mbp1 has been determined by multiwavelength anomalous diffraction from crystals of the selenomethionyl variant of the protein. The structure is composed of a six-stranded beta sheet interspersed with two pairs of alpha helices. The most conserved core region among Mbp1-related transcription factors folds into a central helix-turn-helix motif with a short N-terminal beta strand and a C-terminal beta hairpin. CONCLUSIONS: Despite little sequence similarity, the structure within the core region of the Mbp1 N-terminal domain exhibits a similar fold to that of the DNA-binding domains of other proteins, such as hepatocyte nuclear factor-3gamma and histone H5 from eukaryotes, and the prokaryotic catabolite gene activator. However, the structure outside the core region defines Mbp1 as a larger entity with substructures that stabilize and display the helix-turn-helix motif.
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<StructureSection load='1bm8' size='340' side='right'caption='[[1bm8]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bm8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BM8 FirstGlance]. <br>
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1BM8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BM8 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bm8 OCA], [https://pdbe.org/1bm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bm8 RCSB], [https://www.ebi.ac.uk/pdbsum/1bm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bm8 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis., Xu RM, Koch C, Liu Y, Horton JR, Knapp D, Nasmyth K, Cheng X, Structure. 1997 Mar 15;5(3):349-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9083114 9083114]
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== Function ==
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[https://www.uniprot.org/uniprot/MBP1_YEAST MBP1_YEAST] Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/1bm8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bm8 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Cheng X]]
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[[Category: Cheng, X.]]
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[[Category: Horton JR]]
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[[Category: Horton, J R.]]
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[[Category: Knapp D]]
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[[Category: Knapp, D.]]
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[[Category: Koch C]]
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[[Category: Koch, C.]]
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[[Category: Liu Y]]
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[[Category: Liu, Y.]]
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[[Category: Nasmyth K]]
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[[Category: Nasmyth, K.]]
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[[Category: Xu R-M]]
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[[Category: Xu, R M.]]
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[[Category: cell cycle]]
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[[Category: cyclins]]
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[[Category: dna synthesis]]
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[[Category: helix-turn-helix dna-binding domain]]
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[[Category: multiwavelength anomalous diffraction]]
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[[Category: transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:56:48 2008''
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Current revision

DNA-BINDING DOMAIN OF MBP1

PDB ID 1bm8

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