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1br9

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[[Image:1br9.gif|left|200px]]<br /><applet load="1br9" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1br9, resolution 2.1&Aring;" />
 
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'''HUMAN TISSUE INHIBITOR OF METALLOPROTEINASE-2'''<br />
 
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==Overview==
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==HUMAN TISSUE INHIBITOR OF METALLOPROTEINASE-2==
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The three-dimensional structure of human tissue inhibitor of metalloproteinases-2 (TIMP-2) was determined by X-ray crystallography to 2.1 A resolution. The structure of the inhibitor consists of two domains. The N-terminal domain (residues 1-110) is folded into a beta-barrel, similar to the oligonucleotide/oligosaccharide binding fold otherwise found in certain DNA-binding proteins. The C-terminal domain (residues 111-194) contains a parallel stranded beta-hairpin plus a beta-loop-beta motif. Comparison of the structure of uncomplexed human TIMP-2 with that of bovine TIMP-2 bound to the catalytic domain of human MMP-14 suggests an internal rotation between the two domains of approximately 13 degrees upon binding to the protease. Furthermore, local conformational differences in the two structures that might be induced by formation of the protease-inhibitor complex have been found. The most prominent of these involves residues 27-40 of the A-B beta-hairpin loop. Structure-based alignment of amino acid sequences of representatives of the TIMP family maps the sequence differences mainly to loop regions, and some of these differences are proposed to be responsible for the particular properties of the various TIMP species.
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<StructureSection load='1br9' size='340' side='right'caption='[[1br9]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1br9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BR9 FirstGlance]. <br>
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1BR9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BR9 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1br9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1br9 OCA], [https://pdbe.org/1br9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1br9 RCSB], [https://www.ebi.ac.uk/pdbsum/1br9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1br9 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Three-dimensional structure of human tissue inhibitor of metalloproteinases-2 at 2.1 A resolution., Tuuttila A, Morgunova E, Bergmann U, Lindqvist Y, Maskos K, Fernandez-Catalan C, Bode W, Tryggvason K, Schneider G, J Mol Biol. 1998 Dec 11;284(4):1133-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9837731 9837731]
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== Function ==
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[https://www.uniprot.org/uniprot/TIMP2_HUMAN TIMP2_HUMAN] Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.<ref>PMID:2793861</ref> <ref>PMID:2554304</ref> <ref>PMID:11710594</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/1br9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1br9 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bergmann, U.]]
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[[Category: Bergmann U]]
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[[Category: Lindqvist, Y.]]
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[[Category: Lindqvist Y]]
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[[Category: Morgunova, E.]]
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[[Category: Morgunova E]]
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[[Category: Schneider, G.]]
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[[Category: Schneider G]]
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[[Category: Tryggvason, K.]]
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[[Category: Tryggvason K]]
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[[Category: Tuuttila, A.]]
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[[Category: Tuuttila A]]
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[[Category: proteinase inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:15 2008''
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Current revision

HUMAN TISSUE INHIBITOR OF METALLOPROTEINASE-2

PDB ID 1br9

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