1jqs

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[[Image:1jqs.png|left|200px]]
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==Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog==
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<StructureSection load='1jqs' size='340' side='right' caption='[[1jqs]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jqs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JQS FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1eg0|1eg0]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqs OCA], [http://pdbe.org/1jqs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jqs RCSB], [http://www.ebi.ac.uk/pdbsum/1jqs PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA]] This protein binds directly to 23S ribosomal RNA. [[http://www.uniprot.org/uniprot/EFG_THETH EFG_THETH]] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/1jqs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jqs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation.
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{{STRUCTURE_1jqs| PDB=1jqs | SCENE= }}
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Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation.,Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530<ref>PMID:11518530</ref>
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===Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_11518530}}
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<div class="pdbe-citations 1jqs" style="background-color:#fffaf0;"></div>
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==About this Structure==
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[[1jqs]] is a 3 chain structure of [[Ribosomal protein L11]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQS OCA].
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==See Also==
==See Also==
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*[[Elongation factor|Elongation factor]]
*[[Ribosomal protein L11|Ribosomal protein L11]]
*[[Ribosomal protein L11|Ribosomal protein L11]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011518530</ref><ref group="xtra">PMID:015103642</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Agrawal, R K.]]
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[[Category: Agrawal, R K]]
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[[Category: Frank, J.]]
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[[Category: Frank, J]]
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[[Category: Linde, J.]]
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[[Category: Linde, J]]
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[[Category: Nierhaus, K H.]]
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[[Category: Nierhaus, K H]]
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[[Category: Segupta, J.]]
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[[Category: Segupta, J]]
[[Category: 70s e coli ribosome]]
[[Category: 70s e coli ribosome]]
[[Category: Cryo-em]]
[[Category: Cryo-em]]

Current revision

Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog

1jqs

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