3o0r

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[[Image:3o0r.png|left|200px]]
 
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{{STRUCTURE_3o0r| PDB=3o0r | SCENE= }}
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==Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment==
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<StructureSection load='3o0r' size='340' side='right'caption='[[3o0r]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3o0r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O0R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o0r OCA], [https://pdbe.org/3o0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o0r RCSB], [https://www.ebi.ac.uk/pdbsum/3o0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o0r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NORB_PSEAE NORB_PSEAE] Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen reduction is probably coupled to electron transport phosphorylation (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nitric oxide reductase (NOR) is an iron-containing enzyme that catalyzes the reduction of nitric oxide (NO) to generate a major greenhouse gas, nitrous oxide (N(2)O). Here, we report the crystal structure of NOR from Pseudomonas aeruginosa at 2.7 angstrom resolution. The structure reveals details of the catalytic binuclear center. The non-heme iron (Fe(B)) is coordinated by three His and one Glu ligands, but a His-Tyr covalent linkage common in cytochrome oxidases (COX) is absent. This structural characteristic is crucial for NOR reaction. Although the overall structure of NOR is closely related to COX, neither the D- nor K-proton pathway, which connect the COX active center to the intracellular space, was observed. Protons required for the NOR reaction are probably provided from the extracellular side.
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===Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment===
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Structural basis of biological N2O generation by bacterial nitric oxide reductase.,Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, Iwata S, Shiro Y Science. 2010 Dec 17;330(6011):1666-70. Epub 2010 Nov 25. PMID:21109633<ref>PMID:21109633</ref>
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{{ABSTRACT_PUBMED_21109633}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3o0r" style="background-color:#fffaf0;"></div>
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[[3o0r]] is a 4 chain structure of [[Antibody]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0R OCA].
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==See Also==
==See Also==
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*[[Antibody|Antibody]]
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Cytochrome c nitrite reductase|Cytochrome c nitrite reductase]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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<ref group="xtra">PMID:021109633</ref><references group="xtra"/>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Nitric-oxide reductase]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Fukumori Y]]
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[[Category: Fukumori, Y.]]
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[[Category: Hino T]]
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[[Category: Hino, T.]]
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[[Category: Iwata S]]
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[[Category: Iwata, S.]]
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[[Category: Matsumoto Y]]
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[[Category: Matsumoto, Y.]]
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[[Category: Murata T]]
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[[Category: Murata, T.]]
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[[Category: Nagano S]]
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[[Category: Nagano, S.]]
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[[Category: Shiro Y]]
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[[Category: Shiro, Y.]]
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[[Category: Sugimoto H]]
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[[Category: Sugimoto, H.]]
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[[Category: Cytoplasmic membrane]]
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[[Category: Electron transport]]
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[[Category: Heme]]
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[[Category: Immune system-oxidoreductase complex]]
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[[Category: Iron]]
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[[Category: Membrane]]
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[[Category: Oxidoreductase]]
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Current revision

Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment

PDB ID 3o0r

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