2o4v

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[[Image:2o4v.png|left|200px]]
 
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{{STRUCTURE_2o4v| PDB=2o4v | SCENE= }}
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==An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane==
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<StructureSection load='2o4v' size='340' side='right'caption='[[2o4v]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2o4v]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O4V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o4v OCA], [https://pdbe.org/2o4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o4v RCSB], [https://www.ebi.ac.uk/pdbsum/2o4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o4v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PORP_PSEAE PORP_PSEAE] Anion specific, the binding site has higher affinity for phosphate than chloride ions. Porin O has a higher affinity for polyphosphates (tripolyphosphate and pyrophosphate) while porin P has a higher affinity for orthophosphate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o4/2o4v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o4v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The outer membrane protein OprP mediates the transport of essential phosphate anions into the pathogenic bacterium Pseudomonas aeruginosa. Here we report the crystallographic structure of trimeric OprP at 1.9-A resolution, revealing an unprecedented 9-residue arginine 'ladder' that spans from the extracellular surface down through a constriction zone where phosphate is coordinated. Lysine residues coat the inner periplasmic surface, creating an 'electropositive sink' that pulls the phosphates through the eyelet and into the cell.
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===An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane===
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An arginine ladder in OprP mediates phosphate-specific transfer across the outer membrane.,Moraes TF, Bains M, Hancock RE, Strynadka NC Nat Struct Mol Biol. 2007 Jan;14(1):85-7. Epub 2006 Dec 24. PMID:17187075<ref>PMID:17187075</ref>
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{{ABSTRACT_PUBMED_17187075}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2o4v" style="background-color:#fffaf0;"></div>
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[[2o4v]] is a 3 chain structure of [[Porin]] with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O4V OCA].
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==See Also==
==See Also==
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*[[Porin|Porin]]
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*[[Porin 3D structures|Porin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017187075</ref><references group="xtra"/>
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__TOC__
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[[Category: Pseudomonas aeruginosa pao1]]
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</StructureSection>
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[[Category: Bains, M.]]
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[[Category: Large Structures]]
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[[Category: Hancock, R E.]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Moraes, T F.]]
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[[Category: Bains M]]
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[[Category: Strynadka, N C.]]
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[[Category: Hancock RE]]
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[[Category: Channel]]
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[[Category: Moraes TF]]
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[[Category: Membrane protein]]
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[[Category: Strynadka NC]]
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[[Category: Outer membrane]]
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[[Category: Phosphate]]
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[[Category: Porin]]
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[[Category: Transport]]
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Current revision

An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane

PDB ID 2o4v

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