2pe5

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[[Image:2pe5.png|left|200px]]
 
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{{STRUCTURE_2pe5| PDB=2pe5 | SCENE= }}
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==Crystal Structure of the Lac Repressor bound to ONPG in repressed state==
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<StructureSection load='2pe5' size='340' side='right'caption='[[2pe5]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pe5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PE5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=145:1-O-[O-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>145</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pe5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pe5 OCA], [https://pdbe.org/2pe5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pe5 RCSB], [https://www.ebi.ac.uk/pdbsum/2pe5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pe5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pe/2pe5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pe5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The lac operon is a model system for understanding how effector molecules regulate transcription and are necessary for allosteric transitions. The crystal structures of the lac repressor bound to inducer and anti-inducer molecules provide a model for how these small molecules can modulate repressor function. The structures of the apo repressor and the repressor bound to effector molecules are compared in atomic detail. All effectors examined here bind to the repressor in the same location and are anchored to the repressor through hydrogen bonds to several hydroxyl groups of the sugar ring. Inducer molecules form a more extensive hydrogen-bonding network compared to anti-inducers and neutral effector molecules. The structures of these effector molecules suggest that the O6 hydroxyl on the galactoside is essential for establishing a water-mediated hydrogen bonding network that bridges the N-terminal and C-terminal sub-domains. The altered hydrogen bonding can account in part for the different structural conformations of the repressor, and is vital for the allosteric transition.
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===Crystal Structure of the Lac Repressor bound to ONPG in repressed state===
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Structural analysis of lac repressor bound to allosteric effectors.,Daber R, Stayrook S, Rosenberg A, Lewis M J Mol Biol. 2007 Jul 20;370(4):609-19. Epub 2007 Apr 19. PMID:17543986<ref>PMID:17543986</ref>
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{{ABSTRACT_PUBMED_17543986}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2pe5" style="background-color:#fffaf0;"></div>
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[[2pe5]] is a 6 chain structure of [[Lac repressor]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PE5 OCA].
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==See Also==
==See Also==
*[[Lac repressor|Lac repressor]]
*[[Lac repressor|Lac repressor]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017543986</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Daber, R.]]
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[[Category: Large Structures]]
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[[Category: Lewis, M.]]
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[[Category: Synthetic construct]]
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[[Category: Rosenberg, A.]]
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[[Category: Daber R]]
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[[Category: Stayrook, S E.]]
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[[Category: Lewis M]]
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[[Category: Allosteric effector]]
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[[Category: Rosenberg A]]
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[[Category: Dna-binding]]
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[[Category: Stayrook SE]]
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[[Category: Gene regulation]]
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[[Category: Lac repressor]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transcription-dna complex]]
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Current revision

Crystal Structure of the Lac Repressor bound to ONPG in repressed state

PDB ID 2pe5

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