3t4m

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (02:26, 21 November 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3t4m.png|left|200px]]
 
-
{{STRUCTURE_3t4m| PDB=3t4m | SCENE= }}
+
==Ac-AChBP ligand binding domain mutated to human alpha-7 nAChR (intermediate)==
 +
<StructureSection load='3t4m' size='340' side='right'caption='[[3t4m]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3t4m]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T4M FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t4m OCA], [https://pdbe.org/3t4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t4m RCSB], [https://www.ebi.ac.uk/pdbsum/3t4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t4m ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q8WSF8_APLCA Q8WSF8_APLCA]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Determining the structure of the ligand-binding domain of the nicotinic acetylcholine receptor (nAChR) has been a long standing goal in the design of selective drugs useful in implicated diseases for this prevalent receptor family. Acetylcholine-binding proteins have proven to be valuable surrogates with structural similarity and sequence identity to the extracellular domain of the nicotinic receptor, yet these soluble proteins have their unique features and do not serve as exact replicates of the nAChRs of interest. Here we systematically modify the sequence of these proteins toward the homomeric human alpha7 nAChR. These chimeric proteins exhibit a shift in affinities to reflect alpha7 binding characteristics yet maintain expression levels and stability conducive for crystallization. We also present a pentameric humanoid nAChR extracellular domain with the structural determination of the alpha7 nAChR glycosylation site.
-
===Ac-AChBP ligand binding domain mutated to human alpha-7 nAChR (intermediate)===
+
Creating an alpha7 nicotinic acetylcholine recognition domain from the acetylcholine-binding protein: crystallographic and ligand selectivity analyses.,Nemecz A, Taylor P J Biol Chem. 2011 Dec 9;286(49):42555-65. Epub 2011 Oct 18. PMID:22009746<ref>PMID:22009746</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
==About this Structure==
+
</div>
-
[[3t4m]] is a 10 chain structure of [[Acetylcholine binding protein]] with sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T4M OCA].
+
<div class="pdbe-citations 3t4m" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
-
*[[Acetylcholine binding protein|Acetylcholine binding protein]]
+
*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
-
[[Category: Aplysia californica]]
+
== References ==
-
[[Category: Nemecz, A.]]
+
<references/>
-
[[Category: Taylor, P W.]]
+
__TOC__
-
[[Category: Acetylcholine]]
+
</StructureSection>
-
[[Category: Achbp]]
+
-
[[Category: Alpha-7 human nicotinic acetylcholine receptor]]
+
[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
-
[[Category: Binding protein]]
+
[[Category: Large Structures]]
-
[[Category: Glycosylation]]
+
[[Category: Nemecz A]]
-
[[Category: Mutated acetylcholine binding protein]]
+
[[Category: Taylor PW]]
-
[[Category: Nachr]]
+
-
[[Category: Receptor]]
+

Current revision

Ac-AChBP ligand binding domain mutated to human alpha-7 nAChR (intermediate)

PDB ID 3t4m

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3t4m"
Personal tools