3nfg

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[[Image:3nfg.png|left|200px]]
 
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{{STRUCTURE_3nfg| PDB=3nfg | SCENE= }}
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==Crystal structure of Dimerization module of RNA polymerase I subcomplex A49/A34.5==
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<StructureSection load='3nfg' size='340' side='right'caption='[[3nfg]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nfg]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_glabrata Candida glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NFG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nfg OCA], [https://pdbe.org/3nfg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nfg RCSB], [https://www.ebi.ac.uk/pdbsum/3nfg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nfg ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/3nfg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nfg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related to TFIIF and TFIIE. The N-terminal regions of A49 and A34.5 form a dimerization module that stimulates polymerase-intrinsic RNA cleavage and has a fold that resembles the TFIIF core. The C-terminal region of A49 forms a "tandem winged helix" (tWH) domain that binds DNA with a preference for the upstream promoter nontemplate strand and is predicted in TFIIE. Similar domains are predicted in Pol III-specific subunits. Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity.
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===Crystal structure of Dimerization module of RNA polymerase I subcomplex A49/A34.5===
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RNA polymerase I contains a TFIIF-related DNA-binding subcomplex.,Geiger SR, Lorenzen K, Schreieck A, Hanecker P, Kostrewa D, Heck AJ, Cramer P Mol Cell. 2010 Aug 27;39(4):583-94. PMID:20797630<ref>PMID:20797630</ref>
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{{ABSTRACT_PUBMED_20797630}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3nfg" style="background-color:#fffaf0;"></div>
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[[3nfg]] is a 16 chain structure of [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Candida_glabrata Candida glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NFG OCA].
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== References ==
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<references/>
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==See Also==
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__TOC__
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*[[RNA polymerase|RNA polymerase]]
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</StructureSection>
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[[Category: Large Structures]]
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==Reference==
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[[Category: Geiger SR]]
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<ref group="xtra">PMID:020797630</ref><references group="xtra"/>
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[[Category: Candida glabrata]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Geiger, S R.]]
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[[Category: Dimerization]]
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[[Category: Rna polymerase]]
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[[Category: Transcription]]
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[[Category: Triple barrel]]
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Current revision

Crystal structure of Dimerization module of RNA polymerase I subcomplex A49/A34.5

PDB ID 3nfg

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