2b3y

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[[Image:2b3y.png|left|200px]]
 
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{{STRUCTURE_2b3y| PDB=2b3y | SCENE= }}
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==Structure of a monoclinic crystal form of human cytosolic aconitase (IRP1)==
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<StructureSection load='2b3y' size='340' side='right'caption='[[2b3y]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2b3y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The May 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aconitase and Iron Regulatory Protein 1'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_5 10.2210/rcsb_pdb/mom_2007_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3Y FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3y OCA], [https://pdbe.org/2b3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3y RCSB], [https://www.ebi.ac.uk/pdbsum/2b3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACOHC_HUMAN ACOHC_HUMAN] Bifunctional iron sensor that switches between 2 activities depending on iron availability (PubMed:1946430, PubMed:1281544, PubMed:8041788). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed:1946430, PubMed:1281544, PubMed:8041788, PubMed:23891004). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed:1946430, PubMed:1281544, PubMed:8041788, PubMed:23891004).<ref>PMID:1281544</ref> <ref>PMID:1946430</ref> <ref>PMID:23891004</ref> <ref>PMID:8041788</ref> Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.<ref>PMID:1281544</ref> <ref>PMID:1946430</ref> <ref>PMID:8041788</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3y ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Iron regulatory proteins (IRPs) control the translation of proteins involved in iron uptake, storage and utilization by binding to specific noncoding sequences of the corresponding mRNAs known as iron-responsive elements (IREs). This strong interaction assures proper iron homeostasis in animal cells under iron shortage. Conversely, under iron-replete conditions, IRP1 binds a [4Fe-4S] cluster and functions as cytosolic aconitase. Regulation of the balance between the two IRP1 activities is complex, and it does not depend only on iron availability. Here, we report the crystal structure of human IRP1 in its aconitase form. Comparison with known structures of homologous enzymes reveals well-conserved folds and active site environments with significantly different surface shapes and charge distributions. The specific features of human IRP1 allow us to propose a tentative model of an IRP1-IRE complex that agrees with a range of previously obtained data.
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===Structure of a monoclinic crystal form of human cytosolic aconitase (IRP1)===
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Crystal structure of human iron regulatory protein 1 as cytosolic aconitase.,Dupuy J, Volbeda A, Carpentier P, Darnault C, Moulis JM, Fontecilla-Camps JC Structure. 2006 Jan;14(1):129-39. PMID:16407072<ref>PMID:16407072</ref>
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{{ABSTRACT_PUBMED_16407072}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2b3y" style="background-color:#fffaf0;"></div>
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[[2b3y]] is a 2 chain structure of [[Aconitase]] and [[Samer Kawak Sandbox 4]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The May 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aconitase and Iron Regulatory Protein 1'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_5 10.2210/rcsb_pdb/mom_2007_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3Y OCA].
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==See Also==
==See Also==
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*[[Aconitase|Aconitase]]
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*[[Aconitase 3D structures|Aconitase 3D structures]]
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*[[Samer Kawak Sandbox 4|Samer Kawak Sandbox 4]]
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:016407072</ref><references group="xtra"/>
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</StructureSection>
[[Category: Aconitase and Iron Regulatory Protein 1]]
[[Category: Aconitase and Iron Regulatory Protein 1]]
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[[Category: Aconitate hydratase]]
 
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Dupuy, J.]]
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[[Category: Dupuy J]]
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[[Category: Fontecilla-Camps, J C.]]
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[[Category: Fontecilla-Camps JC]]
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[[Category: Volbeda, A.]]
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[[Category: Volbeda A]]
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[[Category: Irp1 ire-irp1 aconitase activity]]
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[[Category: Lyase]]
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Current revision

Structure of a monoclinic crystal form of human cytosolic aconitase (IRP1)

PDB ID 2b3y

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