1w60

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[[Image:1w60.png|left|200px]]
 
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{{STRUCTURE_1w60| PDB=1w60 | SCENE= }}
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==NATIVE HUMAN PCNA==
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<StructureSection load='1w60' size='340' side='right'caption='[[1w60]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1w60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W60 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w60 OCA], [https://pdbe.org/1w60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w60 RCSB], [https://www.ebi.ac.uk/pdbsum/1w60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w60 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w6/1w60_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w60 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The interactions between the tumor suppressor protein p21WAF1 and the cyclin-dependent kinase (CDK) complexes and with proliferating cell nuclear antigen (PCNA) regulate and coordinate the processes of cell-cycle progression and DNA replication. We present the x-ray crystal structure of PCNA complexed with a 16-mer peptide related to p21 that binds with a Kd of 100 nM. Two additional crystal structures of native PCNA provide previously undescribed structures of uncomplexed human PCNA and show that significant changes on ligand binding include rigidification of a number of flexible regions on the surface of PCNA. In the competitive binding experiments described here, we show that a 20-mer sequence from p21 can be associated simultaneously with PCNA and CDK/cyclin complexes. A structural model for this quaternary complex is presented in which the C-terminal sequence of p21 acts like double-sided tape and docks to both the PCNA and cyclin molecules. The quaternary complex shows little direct interaction between PCNA and cyclin, giving p21 the role of an adaptor molecule. Taken together, the biochemical and structural results delineate a druggable inhibitor site on the surface of PCNA that may be exploited in the design of peptidomimetics, which will act independently of cyclin-groove inhibitors.
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===NATIVE HUMAN PCNA===
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Structural and biochemical studies of human proliferating cell nuclear antigen complexes provide a rationale for cyclin association and inhibitor design.,Kontopidis G, Wu SY, Zheleva DI, Taylor P, McInnes C, Lane DP, Fischer PM, Walkinshaw MD Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1871-6. Epub 2005 Jan 28. PMID:15681588<ref>PMID:15681588</ref>
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{{ABSTRACT_PUBMED_15681588}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1w60" style="background-color:#fffaf0;"></div>
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[[1w60]] is a 2 chain structure of [[Proliferating Cell Nuclear Antigen]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W60 OCA].
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==See Also==
==See Also==
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*[[Proliferating Cell Nuclear Antigen|Proliferating Cell Nuclear Antigen]]
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*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015681588</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Fischer, P.]]
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[[Category: Large Structures]]
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[[Category: Kontopidis, G.]]
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[[Category: Fischer P]]
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[[Category: Lane, D.]]
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[[Category: Kontopidis G]]
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[[Category: Mcinnes, C.]]
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[[Category: Lane D]]
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[[Category: Taylor, P.]]
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[[Category: Mcinnes C]]
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[[Category: Walkinshaw, M.]]
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[[Category: Taylor P]]
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[[Category: Wu, S.]]
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[[Category: Walkinshaw M]]
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[[Category: Zheleva, D.]]
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[[Category: Wu S]]
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[[Category: Dna]]
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[[Category: Zheleva D]]
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[[Category: Dna replication]]
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[[Category: Dna-binding protein]]
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[[Category: Dna-binding systemic lupus erythematosus]]
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[[Category: Oncogene]]
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[[Category: Processivity]]
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[[Category: Replication]]
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Current revision

NATIVE HUMAN PCNA

PDB ID 1w60

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