1cun

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[[Image:1cun.gif|left|200px]]<br /><applet load="1cun" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cun, resolution 2.00&Aring;" />
 
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'''CRYSTAL STRUCTURE OF REPEATS 16 AND 17 OF CHICKEN BRAIN ALPHA SPECTRIN'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF REPEATS 16 AND 17 OF CHICKEN BRAIN ALPHA SPECTRIN==
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Spectrin is a vital component of the cytoskeleton, conferring flexibility on cells and providing a scaffold for a variety of proteins. It is composed of tandem, antiparallel coiled-coil repeats. We report four related crystal structures at 1.45 A, 2.0 A, 3.1 A, and 4.0 A resolution of two connected repeats of chicken brain alpha-spectrin. In all of the structures, the linker region between adjacent units is alpha-helical without breaks, kinks, or obvious boundaries. Two features observed in the structures are (1) conformational rearrangement in one repeat, resulting in movement of the position of a loop, and (2) varying degrees of bending at the linker region. These features form the basis of two different models of flexibility: a conformational rearrangement and a bending model. These models provide novel atomic details of spectrin flexibility.
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<StructureSection load='1cun' size='340' side='right'caption='[[1cun]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cun]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cun OCA], [https://pdbe.org/1cun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cun RCSB], [https://www.ebi.ac.uk/pdbsum/1cun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cun ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cun_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cun ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CUN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUN OCA].
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*[[Spectrin 3D structures|Spectrin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structures of two repeats of spectrin suggest models of flexibility., Grum VL, Li D, MacDonald RI, Mondragon A, Cell. 1999 Aug 20;98(4):523-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10481916 10481916]
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Grum, V L.]]
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[[Category: Grum VL]]
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[[Category: Li, D.]]
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[[Category: Li D]]
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[[Category: MacDonald, R I.]]
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[[Category: MacDonald RI]]
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[[Category: Mondragon, A.]]
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[[Category: Mondragon A]]
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[[Category: 2 tandem 3-helix coiled- coils]]
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[[Category: alpha helical linker region]]
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[[Category: structural protein]]
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[[Category: two repeats of spectrin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:09:52 2008''
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Current revision

CRYSTAL STRUCTURE OF REPEATS 16 AND 17 OF CHICKEN BRAIN ALPHA SPECTRIN

PDB ID 1cun

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