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2y8i

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[[Image:2y8i.png|left|200px]]
 
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{{STRUCTURE_2y8i| PDB=2y8i | SCENE= }}
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==Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2==
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<StructureSection load='2y8i' size='340' side='right'caption='[[2y8i]], [[Resolution|resolution]] 3.13&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2y8i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.132&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8i OCA], [https://pdbe.org/2y8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8i RCSB], [https://www.ebi.ac.uk/pdbsum/2y8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures.
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===STRUCTURAL BASIS FOR THE ALLOSTERIC INTERFERENCE OF MYOSIN FUNCTION BY MUTANTS G680A AND G680V OF DICTYOSTELIUM MYOSIN-2===
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Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195<ref>PMID:21841195</ref>
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{{ABSTRACT_PUBMED_21841195}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2y8i" style="background-color:#fffaf0;"></div>
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[[2y8i]] is a 1 chain structure of [[Myosin]] with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8I OCA].
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==See Also==
==See Also==
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*[[Myosin|Myosin]]
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021841195</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
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[[Category: Myosin ATPase]]
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[[Category: Large Structures]]
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[[Category: Adamek, N.]]
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[[Category: Adamek N]]
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[[Category: Bauer, S.]]
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[[Category: Bauer S]]
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[[Category: Fedorov, R.]]
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[[Category: Fedorov R]]
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[[Category: Fujita-Becker, S.]]
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[[Category: Fujita-Becker S]]
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[[Category: Geeves, M A.]]
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[[Category: Geeves MA]]
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[[Category: Manstein, D J.]]
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[[Category: Manstein DJ]]
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[[Category: Preller, M.]]
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[[Category: Preller M]]
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[[Category: Adp complex]]
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[[Category: Motor protein]]
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Current revision

Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2

PDB ID 2y8i

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