This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ibc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:01, 3 April 2024) (edit) (undo)
 
(23 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ibc.gif|left|200px]]<br />
 
-
<applet load="1ibc" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ibc, resolution 2.73&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF INHIBITED INTERLEUKIN-1BETA CONVERTING ENZYME'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF INHIBITED INTERLEUKIN-1BETA CONVERTING ENZYME==
-
BACKGROUND: Interleukin-1beta converting enzyme (ICE/caspase-1) is the, protease responsible for interleukin-1beta (IL-1beta) production in, monocytes. It was the first member of a new cysteine protease family to be, identified. Members of this family have functions in both inflammation and, apoptosis. RESULTS: A novel method for identifying protease specificity, employing a positional-scanning substrate library, was used to determine, the amino-acid preferences of ICE. Using this method, the complete, specificity of a protease can be mapped in the time required to perform, one assay. The results indicate that the optimal tetrapeptide recognition, sequence for ICE is WEHD, not YVAD, as previously believed, and this led, to the synthesis of an unusually potent aldehyde inhibitor, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9190289 (full description)]]
+
<StructureSection load='1ibc' size='340' side='right'caption='[[1ibc]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ibc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IBC FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.73&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ASA:ASPARTIC+ALDEHYDE'>ASA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ibc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ibc OCA], [https://pdbe.org/1ibc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ibc RCSB], [https://www.ebi.ac.uk/pdbsum/1ibc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ibc ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CASP1_HUMAN CASP1_HUMAN] Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.<ref>PMID:7876192</ref> <ref>PMID:15498465</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ib/1ibc_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ibc ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1IBC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with ACE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Caspase-1 Caspase-1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.36 3.4.22.36]]. Structure known Active Sites: S1, S2, S3 and S4. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IBC OCA]].
+
*[[Caspase 3D structures|Caspase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
A combinatorial approach for determining protease specificities: application to interleukin-1beta converting enzyme (ICE)., Rano TA, Timkey T, Peterson EP, Rotonda J, Nicholson DW, Becker JW, Chapman KT, Thornberry NA, Chem Biol. 1997 Feb;4(2):149-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9190289 9190289]
+
__TOC__
-
[[Category: Caspase-1]]
+
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Becker, J.W.]]
+
[[Category: Becker JW]]
-
[[Category: Rotonda, J.]]
+
[[Category: Rotonda J]]
-
[[Category: ACE]]
+
-
[[Category: caspase-1]]
+
-
[[Category: complex (hydrolase/peptide)]]
+
-
[[Category: cysteine protease]]
+
-
[[Category: ice]]
+
-
[[Category: interleukin-1beta converting enzyme]]
+
-
[[Category: protease]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:48:57 2007''
+

Current revision

CRYSTAL STRUCTURE OF INHIBITED INTERLEUKIN-1BETA CONVERTING ENZYME

PDB ID 1ibc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ibc"
Personal tools