3fd2

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[[Image:3fd2.png|left|200px]]
 
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{{STRUCTURE_3fd2| PDB=3fd2 | SCENE= }}
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==Crystal structure of mMsoI/DNA complex with calcium==
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<StructureSection load='3fd2' size='340' side='right'caption='[[3fd2]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fd2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Monomastix_sp._OKE-1 Monomastix sp. OKE-1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FD2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fd2 OCA], [https://pdbe.org/3fd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fd2 RCSB], [https://www.ebi.ac.uk/pdbsum/3fd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fd2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/C0JWR6_MONSK C0JWR6_MONSK]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fd2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fd2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Homing endonucleases (HEs) cut long DNA target sites with high specificity to initiate and target the lateral transfer of mobile introns or inteins. This high site specificity of HEs makes them attractive reagents for gene targeting to promote DNA modification or repair. We have generated several hundred catalytically active, monomerized versions of the well-characterized homodimeric I-CreI and I-MsoI LAGLIDADG family homing endonuclease (LHE) proteins. Representative monomerized I-CreI and I-MsoI proteins (collectively termed mCreIs or mMsoIs) were characterized in detail by using a combination of biochemical, biophysical and structural approaches. We also demonstrated that both mCreI and mMsoI proteins can promote cleavage-dependent recombination in human cells. The use of single chain LHEs should simplify gene modification and targeting by requiring the expression of a single small protein in cells, rather than the coordinate expression of two separate protein coding genes as is required when using engineered heterodimeric zinc finger or homing endonuclease proteins.
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===Crystal structure of mMsoI/DNA complex with calcium===
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Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins.,Li H, Pellenz S, Ulge U, Stoddard BL, Monnat RJ Jr Nucleic Acids Res. 2009 Apr;37(5):1650-62. Epub 2009 Jan 19. PMID:19153140<ref>PMID:19153140</ref>
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{{ABSTRACT_PUBMED_19153140}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3fd2" style="background-color:#fffaf0;"></div>
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[[3fd2]] is a 3 chain structure of [[Endonuclease]] with sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD2 OCA].
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==See Also==
==See Also==
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*[[Endonuclease|Endonuclease]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019153140</ref><references group="xtra"/>
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__TOC__
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[[Category: Eukaryota]]
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</StructureSection>
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[[Category: Li, H.]]
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[[Category: Large Structures]]
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[[Category: Monnat, R J.]]
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[[Category: Monomastix sp. OKE-1]]
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[[Category: Chloroplast]]
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[[Category: Synthetic construct]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Li H]]
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[[Category: Protein-dna complex]]
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[[Category: Monnat RJ]]

Current revision

Crystal structure of mMsoI/DNA complex with calcium

PDB ID 3fd2

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