1d8h

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[[Image:1d8h.gif|left|200px]]<br /><applet load="1d8h" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1d8h, resolution 2.00&Aring;" />
 
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'''X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.'''<br />
 
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==Overview==
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==X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.==
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RNA triphosphatase is an essential mRNA processing enzyme that catalyzes the first step in cap formation. The 2.05 A crystal structure of yeast RNA triphosphatase Cet1p reveals a novel active site fold whereby an eight-stranded beta barrel forms a topologically closed triphosphate tunnel. Interactions of a sulfate in the center of the tunnel with a divalent cation and basic amino acids projecting into the tunnel suggest a catalytic mechanism that is supported by mutational data. Discrete surface domains mediate Cet1p homodimerization and Cet1p binding to the guanylyltransferase component of the capping apparatus. The structure and mechanism of fungal RNA triphosphatases are completely different from those of mammalian mRNA capping enzymes. Hence, RNA triphosphatase presents an ideal target for structure-based antifungal drug discovery.
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<StructureSection load='1d8h' size='340' side='right'caption='[[1d8h]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1d8h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D8H FirstGlance]. <br>
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1D8H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_5'-phosphatase Polynucleotide 5'-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.33 3.1.3.33] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8H OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d8h OCA], [https://pdbe.org/1d8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d8h RCSB], [https://www.ebi.ac.uk/pdbsum/1d8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d8h ProSAT]</span></td></tr>
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Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus., Lima CD, Wang LK, Shuman S, Cell. 1999 Nov 24;99(5):533-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10589681 10589681]
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</table>
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[[Category: Polynucleotide 5'-phosphatase]]
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== Function ==
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[https://www.uniprot.org/uniprot/CET1_YEAST CET1_YEAST] First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.<ref>PMID:12788946</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d8/1d8h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d8h ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Lima CD]]
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[[Category: Lima, C D.]]
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[[Category: Shuman S]]
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[[Category: Shuman, S.]]
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[[Category: Wang LK]]
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[[Category: Wang, L K.]]
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[[Category: MN]]
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[[Category: SO4]]
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[[Category: beta subunit]]
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[[Category: catalytic domain]]
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[[Category: dimer]]
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[[Category: manganese/sulfate complex]]
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[[Category: mrna capping]]
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[[Category: mrna processing]]
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[[Category: nuclear protein beta barrel]]
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[[Category: polynucleotide 5'-triphosphatase]]
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[[Category: rna triphosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:04 2008''
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Current revision

X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.

PDB ID 1d8h

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