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1pif

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[[Image:1pif.gif|left|200px]]<br />
 
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<applet load="1pif" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pif, resolution 2.3&Aring;" />
 
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'''PIG ALPHA-AMYLASE'''<br />
 
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==Overview==
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==PIG ALPHA-AMYLASE==
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The crystal structures of porcine pancreatic alpha-amylase isozyme II (PPA, II) in its free form and complexed with the trestatin A derived, pseudo-octasaccharide V-1532 have been determined using Patterson search, techniques at resolutions of 2.3 and 2.2 angstroms, respectively. Seven, rings of the competitive inhibitor V-1532 could be detected in the active, site region as well as two maltose units in secondary binding sites on the, surface. V-1532 occupies the five central sugar binding subsites similar, to the PPA/acarbose structure. A sixth ring exists at the reducing end, connecting two symmetry related PPA molecules. The seventh moiety, a, 6-hydroxymethylconduritol ring, is located at the non-reducing end. The, electron density for this ring is relatively weak, indicating ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8757803 (full description)]]
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<StructureSection load='1pif' size='340' side='right'caption='[[1pif]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PIF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pif OCA], [https://pdbe.org/1pif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pif RCSB], [https://www.ebi.ac.uk/pdbsum/1pif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pif ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMYP_PIG AMYP_PIG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/1pif_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pif ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PIF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Structure known Active Sites: AS, CA and CL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PIF OCA]].
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*[[Amylase 3D structures|Amylase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Carbohydrate and protein-based inhibitors of porcine pancreatic alpha-amylase: structure analysis and comparison of their binding characteristics., Machius M, Vertesy L, Huber R, Wiegand G, J Mol Biol. 1996 Jul 19;260(3):409-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8757803 8757803]
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[[Category: Large Structures]]
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[[Category: Alpha-amylase]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Machius, M.]]
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[[Category: Machius M]]
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[[Category: Vertesy, L.]]
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[[Category: Vertesy L]]
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[[Category: Wiegand, G.]]
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[[Category: Wiegand G]]
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[[Category: CA]]
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[[Category: CL]]
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[[Category: 4-glucan-4-glucanohydrolase glycosyltransferase]]
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[[Category: alpha-amylase alpha-1]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:49:43 2007''
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Current revision

PIG ALPHA-AMYLASE

PDB ID 1pif

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