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2x8r

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[[Image:2x8r.png|left|200px]]
 
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{{STRUCTURE_2x8r| PDB=2x8r | SCENE= }}
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==The structure of a family GH25 lysozyme from Aspergillus fumigatus==
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<StructureSection load='2x8r' size='340' side='right'caption='[[2x8r]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2x8r]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X8R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8r OCA], [https://pdbe.org/2x8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x8r RCSB], [https://www.ebi.ac.uk/pdbsum/2x8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x8r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A4DA29_ASPFU A4DA29_ASPFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x8r ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lysins are important biomolecules which cleave the bacterial cell-wall polymer peptidoglycan. They are finding increasing commercial and medical application. In order to gain an insight into the mechanism by which these enzymes operate, the X-ray structure of a CAZy family GH25 ;lysozyme' from Aspergillus fumigatus was determined. This is the first fungal structure from the family and reveals a modified alpha/beta-barrel-like fold in which an eight-stranded beta-barrel is flanked by three alpha-helices. The active site lies toward the bottom of a negatively charged pocket and its layout has much in common with other solved members of the GH25 and related GH families. A conserved active-site DXE motif may be implicated in catalysis, lending further weight to the argument that this glycoside hydrolase family operates via a ;substrate-assisted' catalytic mechanism.
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===The structure of a family GH25 lysozyme from Aspergillus fumigatus===
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The structure of a family GH25 lysozyme from Aspergillus fumigatus.,Korczynska JE, Danielsen S, Schagerlof U, Turkenburg JP, Davies GJ, Wilson KS, Taylor EJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt, 9):973-7. Epub 2010 Aug 21. PMID:20823508<ref>PMID:20823508</ref>
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{{ABSTRACT_PUBMED_20823508}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2x8r" style="background-color:#fffaf0;"></div>
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[[2x8r]] is a 6 chain structure of [[Hen Egg-White (HEW) Lysozyme]] with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8R OCA].
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==See Also==
==See Also==
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*[[Hen Egg-White (HEW) Lysozyme|Hen Egg-White (HEW) Lysozyme]]
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020823508</ref><references group="xtra"/>
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__TOC__
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[[Category: Aspergillus fumigatus]]
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</StructureSection>
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[[Category: Lysozyme]]
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[[Category: Aspergillus fumigatus Af293]]
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[[Category: Danielsen, S.]]
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[[Category: Large Structures]]
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[[Category: Davies, G J.]]
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[[Category: Danielsen S]]
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[[Category: Korczynska, J E.]]
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[[Category: Davies GJ]]
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[[Category: Schagerlof, U.]]
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[[Category: Korczynska JE]]
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[[Category: Taylor, E J.]]
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[[Category: Schagerlof U]]
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[[Category: Turkenburg, J P.]]
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[[Category: Taylor EJ]]
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[[Category: Wilson, K S.]]
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[[Category: Turkenburg JP]]
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[[Category: Dxe motif]]
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[[Category: Wilson KS]]
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[[Category: Endo-n-acetylmuramidase]]
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[[Category: Hydrolase]]
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[[Category: Peptidoglycan cleavage]]
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Current revision

The structure of a family GH25 lysozyme from Aspergillus fumigatus

PDB ID 2x8r

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