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1hbw

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[[Image:1hbw.png|left|200px]]
 
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{{STRUCTURE_1hbw| PDB=1hbw | SCENE= }}
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==Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106)==
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<StructureSection load='1hbw' size='340' side='right'caption='[[1hbw]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HBW FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hbw OCA], [https://pdbe.org/1hbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hbw RCSB], [https://www.ebi.ac.uk/pdbsum/1hbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hbw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAL4_YEAST GAL4_YEAST] This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GAL4 dimerization domain (GAL4-dd) is a powerful transcriptional activator when tethered to DNA in a cell bearing a mutant of the GAL11 protein, named GAL11P. GAL11P (like GAL11) is a component of the RNA-polymerase II holoenzyme. Nuclear magnetic resonance (NMR) studies of GAL4-dd revealed an elongated dimer structure with C(2) symmetry containing three helices that mediate dimerization via coiled-coil contacts. The two loops between the three coiled coils form mobile bulges causing a variation of twist angles between the helix pairs. Chemical shift perturbation analysis mapped the GAL11P-binding site to the C-terminal helix alpha3 and the loop between alpha1 and alpha2. One GAL11P monomer binds to one GAL4-dd dimer rendering the dimer asymmetric and implying an extreme negative cooperativity mechanism. Alanine-scanning mutagenesis of GAL4-dd showed that the NMR-derived GAL11P-binding face is crucial for the novel transcriptional activating function of the GAL4-dd on GAL11P interaction. The binding of GAL4 to GAL11P, although an artificial interaction, represents a unique structural motif for an activating region capable of binding to a single target to effect gene expression.
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===SOLUTION NMR STRUCTURE OF THE DIMERIZATION DOMAIN OF THE YEAST TRANSCRIPTIONAL ACTIVATOR GAL4 (RESIDUES 50-106)===
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Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain.,Hidalgo P, Ansari AZ, Schmidt P, Hare B, Simkovich N, Farrell S, Shin EJ, Ptashne M, Wagner G Genes Dev. 2001 Apr 15;15(8):1007-20. PMID:11316794<ref>PMID:11316794</ref>
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{{ABSTRACT_PUBMED_11316794}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1hbw" style="background-color:#fffaf0;"></div>
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[[1hbw]] is a 2 chain structure of [[Gal4]]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBW OCA].
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==See Also==
==See Also==
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*[[Gal4|Gal4]]
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*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011316794</ref><references group="xtra"/>
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__TOC__
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[[Category: Ansari, A Z.]]
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</StructureSection>
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[[Category: Farrell, S.]]
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[[Category: Large Structures]]
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[[Category: Hare, B.]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Hidalgo, P.]]
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[[Category: Ansari AZ]]
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[[Category: Ptashne, M.]]
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[[Category: Farrell S]]
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[[Category: Schmidt, P.]]
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[[Category: Hare B]]
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[[Category: Shin, E J.]]
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[[Category: Hidalgo P]]
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[[Category: Simkovic, N.]]
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[[Category: Ptashne M]]
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[[Category: Wagner, G.]]
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[[Category: Schmidt P]]
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[[Category: Coiled-coil dimeric]]
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[[Category: Shin EJ]]
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[[Category: Dimerization domain]]
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[[Category: Simkovic N]]
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[[Category: Galactose and melibiose metabolism]]
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[[Category: Wagner G]]
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[[Category: Transcriptional activator]]
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Current revision

Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106)

PDB ID 1hbw

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