3d3c

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[[Image:3d3c.png|left|200px]]
 
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{{STRUCTURE_3d3c| PDB=3d3c | SCENE= }}
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==Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.==
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<StructureSection load='3d3c' size='340' side='right'caption='[[3d3c]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3d3c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D3C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d3c OCA], [https://pdbe.org/3d3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d3c RCSB], [https://www.ebi.ac.uk/pdbsum/3d3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d3c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NUSB_ECOLI NUSB_ECOLI] One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the BoxA RNA motif.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/3d3c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d3c ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein S10 is a component of the 30S ribosomal subunit and participates together with NusB protein in processive transcription antitermination. The molecular mechanisms by which S10 can act as a translation or a transcription factor are not understood. We used complementation assays and recombineering to delineate regions of S10 dispensable for antitermination, and determined the crystal structure of a transcriptionally active NusB-S10 complex. In this complex, S10 adopts the same fold as in the 30S subunit and is blocked from simultaneous association with the ribosome. Mass spectrometric mapping of UV-induced crosslinks revealed that the NusB-S10 complex presents an intermolecular, composite, and contiguous binding surface for RNAs containing BoxA antitermination signals. Furthermore, S10 overproduction complemented a nusB null phenotype. These data demonstrate that S10 and NusB together form a BoxA-binding module, that NusB facilitates entry of S10 into the transcription machinery, and that S10 represents a central hub in processive antitermination.
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===Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.===
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Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.,Luo X, Hsiao HH, Bubunenko M, Weber G, Court DL, Gottesman ME, Urlaub H, Wahl MC Mol Cell. 2008 Dec 26;32(6):791-802. PMID:19111659<ref>PMID:19111659</ref>
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{{ABSTRACT_PUBMED_19111659}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3d3c" style="background-color:#fffaf0;"></div>
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[[3d3c]] is a 6 chain structure of [[Ribosomal protein S10]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D3C OCA].
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==See Also==
==See Also==
*[[Ribosomal protein S10|Ribosomal protein S10]]
*[[Ribosomal protein S10|Ribosomal protein S10]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019111659</ref><references group="xtra"/>
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__TOC__
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[[Category: Escherichia coli]]
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</StructureSection>
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[[Category: Luo, X.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Wahl, M C.]]
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[[Category: Large Structures]]
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[[Category: Lambda n antitermination]]
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[[Category: Luo X]]
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[[Category: Nusb]]
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[[Category: Wahl MC]]
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[[Category: Nuse]]
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[[Category: Nut]]
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[[Category: Phage lambda]]
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[[Category: Ribonucleoprotein]]
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[[Category: Ribosomal protein]]
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[[Category: Rna-binding]]
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[[Category: Rrn antitermination]]
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[[Category: S10]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transcription termination]]
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Current revision

Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.

PDB ID 3d3c

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