1wza

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[[Image:1wza.png|left|200px]]
 
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{{STRUCTURE_1wza| PDB=1wza | SCENE= }}
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==Crystal structure of alpha-amylase from H.orenii==
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<StructureSection load='1wza' size='340' side='right'caption='[[1wza]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wza]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halothermothrix_orenii Halothermothrix orenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WZA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wza OCA], [https://pdbe.org/1wza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wza RCSB], [https://www.ebi.ac.uk/pdbsum/1wza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wza ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GPL8_9FIRM Q8GPL8_9FIRM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/1wza_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wza ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stability at high salinity. Sedimentation velocity and CD experiments on AmyA reveal the formation of unique reversible poly-dispersed oligomers that show unusually high thermal stability. These studies provide valuable insight into the structural elements that contribute to the stability of AmyA at both physical and chemical extremes and their functional implications.
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===Crystal structure of alpha-amylase from H.orenii===
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Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly-extreme condition.,Sivakumar N, Li N, Tang JW, Patel BK, Swaminathan K FEBS Lett. 2006 May 15;580(11):2646-52. Epub 2006 Apr 19. PMID:16647060<ref>PMID:16647060</ref>
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{{ABSTRACT_PUBMED_16647060}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1wza" style="background-color:#fffaf0;"></div>
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[[1wza]] is a 1 chain structure of [[Alpha-Amylase]] with sequence from [http://en.wikipedia.org/wiki/Halothermothrix_orenii Halothermothrix orenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WZA OCA].
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==See Also==
==See Also==
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*[[Alpha-Amylase|Alpha-Amylase]]
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*[[Amylase 3D structures|Amylase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016647060</ref><ref group="xtra">PMID:012177328</ref><references group="xtra"/>
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__TOC__
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[[Category: Alpha-amylase]]
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</StructureSection>
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[[Category: Halothermothrix orenii]]
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[[Category: Li, N.]]
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[[Category: Sivakumar, N.]]
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[[Category: Swaminathan, K.]]
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[[Category: Alpha amylase]]
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[[Category: Halophilic]]
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[[Category: Halothermothrix orenii]]
[[Category: Halothermothrix orenii]]
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[[Category: Hydrolase]]
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[[Category: Large Structures]]
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[[Category: Thermophilic]]
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[[Category: Li N]]
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[[Category: Sivakumar N]]
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[[Category: Swaminathan K]]

Current revision

Crystal structure of alpha-amylase from H.orenii

PDB ID 1wza

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